Document Detail

Regulation and evaluation of five methanol-inducible promoters in the methylotrophic yeast Candida boidinii.
MedLine Citation:
PMID:  10978507     Owner:  NLM     Status:  MEDLINE    
We isolated the promoter regions of five methanol-inducible genes (P(AOD1), alcohol oxidase; P(DAS1), dihydroxyacetone synthase; P(FDH1), formate dehydrogenase; P(PMP20), Pmp20; and P(PMP47), Pmp47) from the Candida boidinii genome, and evaluated their strength and studied their regulation using the acid phosphatase gene of Saccharomyces cerevisiae (ScPHO5) as the reporter. Of the five promoters, P(DAS1) was the strongest methanol-inducible promoter whose strength was approximately 1.5 times higher than that of the commonly used P(AOD1) in methanol-induced cells. Although the expression of P(AOD1) and P(DAS1) was completely repressed by the presence of glucose, formate-induced expression of P(FDH1) was not repressed by glucose. Expression under P(PMP47), another methanol-inducible promoter, was highly induced by oleate. The induction kinetics of P(PMP47) and P(DAS1) revealed that methanol induces the expression of peroxisome membrane protein Pmp47, earlier than the expression of matrix enzyme dihydroxyacetone synthase (Das1p), and that this information is contained in the promoter region of the respective gene. This is the first report which evaluates several methanol-inducible promoters in parallel in the methylotrophic yeast.
H Yurimoto; T Komeda; C R Lim; T Nakagawa; K Kondo; N Kato; Y Sakai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1493     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-11-07     Completed Date:  2000-12-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  56-63     Citation Subset:  IM    
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan.
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MeSH Terms
Acid Phosphatase / analysis,  genetics
Alcohol Oxidoreductases / biosynthesis,  genetics
Aldehyde-Ketone Transferases / biosynthesis,  genetics
Candida / drug effects*,  genetics,  metabolism
Consensus Sequence
Enzyme Induction / drug effects
Formate Dehydrogenases
Fungal Proteins / biosynthesis,  genetics
Gene Expression Regulation, Enzymologic / drug effects*
Genes, Reporter
Membrane Proteins / biosynthesis,  genetics
Methanol / pharmacology*
Polymerase Chain Reaction
Promoter Regions, Genetic / physiology*
Saccharomyces cerevisiae
Sequence Alignment
Reg. No./Substance:
0/Fungal Proteins; 0/Membrane Proteins; 0/PMP47 protein, Candida boidinii; 50-99-7/Glucose; 67-56-1/Methanol; EC 1.1.-/Alcohol Oxidoreductases; EC oxidase; EC 1.11.1.-/Peroxidases; EC; EC protein, Candida; EC Dehydrogenases; EC 2.2.-/Aldehyde-Ketone Transferases; EC transketolase; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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