Document Detail

Regulation of erythrocyte cation and water content in sickle cell anemia.
MedLine Citation:
PMID:  3961486     Owner:  NLM     Status:  MEDLINE    
The pathophysiological events in sickle cell disease are critically dependent on the intracellular concentration of hemoglobin S, which varies inversely with cell cation and water content. Erythrocytes of SS homozygotes exposed to oxygen or carbon monoxide decrease their potassium and water content through a pathway for potassium transport that is activated by both cell swelling and decrease in internal pH. This pathway is not inhibited by ouabain either with or without bumetanide. When SS erythrocytes were separated according to density, the pH- and volume-dependent potassium transport was greatest in the least dense fraction and was reduced in the densest cells. This pathway, which does not depend on polymerization of sickle hemoglobin, may be important in regulating the cation and water content of SS erythrocytes.
C Brugnara; H F Bunn; D C Tosteson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  232     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-04-29     Completed Date:  1986-04-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  388-90     Citation Subset:  IM    
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MeSH Terms
Anemia, Sickle Cell / blood*,  metabolism
Body Water / metabolism*
Bumetanide / pharmacology
Erythrocytes / analysis,  drug effects,  metabolism*,  physiology
Hemoglobin, Sickle / analysis,  metabolism
Hydrogen-Ion Concentration
Ouabain / pharmacology
Potassium / analysis,  metabolism*
Sodium / analysis,  metabolism*
Grant Support
Reg. No./Substance:
0/Hemoglobin, Sickle; 28395-03-1/Bumetanide; 630-60-4/Ouabain; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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