| Regulation of endothelin-converting enzyme 1 in nephrotic syndrome in rats. | |
| | |
MedLine Citation:
|
PMID: 12972712 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Nephrotic syndrome is characterized by severe proteinuria and sodium and water retention. Although endothelin (ET) 1 can cause natriuresis or antinatriuresis, the role played by ET-1 in proteinuria and in sodium retention due to nephrotic syndrome remains unclear. METHODS: We investigated the role played by the ET-1 system in sodium and water retention and in proteinuria in puromycin aminonucleoside induced nephrotic syndrome in rats using microdissected nephron segments, competitive polymerase chain reaction, and Western blot. RESULTS: The expression of prepro ET-1, ET-converting enzyme 1 (ECE-1), and ET A receptor mRNAs, but not ET B receptor mRNA, in the glomeruli was increased in rats with nephrotic syndrome. The cGMP generation in the glomeruli induced by atrial natriuretic peptide and ET-1 was decreased, whereas the ET-3-induced cGMP generation was increased in rats with nephrotic syndrome. ECE-1 mRNA expression was increased not only in the glomeruli, but also in the thick ascending limbs and collecting ducts. The protein expression of ECE-1 was increased in the membrane fraction of the cortex and in the outer and the inner medulla of nephrotic rats. Blockade of ET A and B receptors by bosentan did not inhibit the occurrence of nephrotic syndrome. However, the administration of bosentan increased the urinary sodium excretion. CONCLUSION: These data suggest that an activated ET-1-ET A receptor pathway in glomeruli and/or an increased ECE-1 mRNA expression in distal segments may participate in sodium and water retention, but not in the occurrence of nephrotic syndrome. |
| | |
Authors:
|
Mika Ikebe; Hiroshi Nonoguchi; Yushi Nakayama; Yuka Tashima; Kohei Shimada; Kazuhiko Tanzawa; Kimio Tomita |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Nephron. Experimental nephrology Volume: 94 ISSN: 1660-2129 ISO Abbreviation: Nephron Exp. Nephrol. Publication Date: 2003 |
Date Detail:
|
Created Date: 2003-09-15 Completed Date: 2006-04-04 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 101159770 Medline TA: Nephron Exp Nephrol Country: Switzerland |
Other Details:
|
Languages: eng Pagination: e137-45 Citation Subset: IM |
Copyright Information:
|
Copyright 2003 S. Karger AG, Basel |
Affiliation:
|
Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, Kumamoto, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Aspartic Acid Endopeptidases / genetics, metabolism, physiology* Atrial Natriuretic Factor / metabolism Cyclic GMP / biosynthesis Endothelin-1 / biosynthesis, genetics, metabolism Endothelin-3 / metabolism Enzyme Induction / genetics Gene Expression Regulation, Enzymologic / genetics Kidney Glomerulus / enzymology Male Metalloendopeptidases / genetics, metabolism, physiology* Nephrons / enzymology, metabolism, pathology Nephrotic Syndrome / blood, enzymology*, metabolism, urine Proteinuria / chemically induced Puromycin Aminonucleoside / adverse effects RNA, Messenger / biosynthesis, genetics Rats Rats, Sprague-Dawley Receptors, Endothelin / antagonists & inhibitors Sulfonamides / pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Endothelin-1; 0/Endothelin-3; 0/RNA, Messenger; 0/Receptors, Endothelin; 0/Sulfonamides; 147536-97-8/bosentan; 58-60-6/Puromycin Aminonucleoside; 7665-99-8/Cyclic GMP; 85637-73-6/Atrial Natriuretic Factor; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.71/endothelin-converting enzyme |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: TGF-beta1 induces aberrant laminin chain and collagen type IV isotype expression in the glomerular b...
Next Document: Expression of hepatocyte growth factor is induced by the interaction between human mesangial cells a...