Document Detail


Regulation of dynein localization and centrosome positioning by Lis-1 and asunder during Drosophila spermatogenesis.
MedLine Citation:
PMID:  22764052     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dynein, a microtubule motor complex, plays crucial roles in cell-cycle progression in many systems. The LIS1 accessory protein directly binds dynein, although its precise role in regulating dynein remains unclear. Mutation of human LIS1 causes lissencephaly, a developmental brain disorder. To gain insight into the in vivo functions of LIS1, we characterized a male-sterile allele of the Drosophila homolog of human LIS1. We found that centrosomes do not properly detach from the cell cortex at the onset of meiosis in most Lis-1 spermatocytes; centrosomes that do break cortical associations fail to attach to the nucleus. In Lis-1 spermatids, we observed loss of attachments between the nucleus, basal body and mitochondria. The localization pattern of LIS-1 protein throughout Drosophila spermatogenesis mirrors that of dynein. We show that dynein recruitment to the nuclear surface and spindle poles is severely reduced in Lis-1 male germ cells. We propose that Lis-1 spermatogenesis phenotypes are due to loss of dynein regulation, as we observed similar phenotypes in flies null for Tctex-1, a dynein light chain. We have previously identified asunder (asun) as another regulator of dynein localization and centrosome positioning during Drosophila spermatogenesis. We now report that Lis-1 is a strong dominant enhancer of asun and that localization of LIS-1 in male germ cells is ASUN dependent. We found that Drosophila LIS-1 and ASUN colocalize and coimmunoprecipitate from transfected cells, suggesting that they function within a common complex. We present a model in which Lis-1 and asun cooperate to regulate dynein localization and centrosome positioning during Drosophila spermatogenesis.
Authors:
Poojitha Sitaram; Michael A Anderson; Jeanne N Jodoin; Ethan Lee; Laura A Lee
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-04
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-26     Completed Date:  2012-10-16     Revised Date:  2013-08-18    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2945-54     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, U-4225 Medical Research Building III, 465 21st Avenue South, Nashville, TN 37232-8240, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Cell Cycle Proteins / genetics,  metabolism*
Centrosome / metabolism
Drosophila Proteins / genetics,  metabolism*
Drosophila melanogaster / genetics,  growth & development,  metabolism
Dyneins / genetics,  metabolism*
Humans
Male
Microtubule-Associated Proteins / genetics,  metabolism*
Models, Biological
Phenotype
Spermatids / metabolism,  ultrastructure
Spermatocytes / metabolism,  ultrastructure
Spermatogenesis / genetics,  physiology*
Grant Support
ID/Acronym/Agency:
2T32HD007043/HD/NICHD NIH HHS; 2T32HD007502/HD/NICHD NIH HHS; GM074044/GM/NIGMS NIH HHS; R01 GM074044/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Drosophila Proteins; 0/Lis-1 protein, Drosophila; 0/Microtubule-Associated Proteins; 0/asunder protein, Drosophila; 144198-36-7/dynactin; EC 3.6.4.2/Dyneins
Comments/Corrections

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