Document Detail


Regulation of dopamine transporter expression by neuronal activity.
MedLine Citation:
PMID:  23296787     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Actions of extracellular dopamine released in the central nervous system are primarily terminated by the dopamine transporter. This protein is also a target for therapeutic and abused psychostimulant drugs. Different methods used to study dopamine transporter function, its expression, and intracellular signaling in neurons are described in this chapter. Function of the dopamine transporter in mesencephalic primary cultures can be measured by dopamine uptake assay. Expression of the transporter protein and mRNA are analyzed by western blots and quantitative RT-PCR, respectively. Finally, methods to study neuronal activity-dependent changes in Ca(2+)⁄calmodulin-dependent protein (CaM) kinase activity in dopamine neurons are described.
Authors:
Shalini Padmanabhan; Thach Pham; Balakrishna M Prasad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  964     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  229-42     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Medical College of Georgia, Augusta, GA, USA.
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