Document Detail

Regulation of dome formation in differentiated epithelial cell cultures.
MedLine Citation:
PMID:  232529     Owner:  NLM     Status:  MEDLINE    
Rat mammary (Rama 25) and dog kidney (MDCK) epithelial cell cultures formed 'domes' of cells due to fluid accumulation in focal regions between the culture dish and the cell monolayer. Addition of ouabain caused collapse of domes, suggesting that transport functions were required for maintenance of domes. Dome formation in both epithelial cell lines was stimulated by a broad spectrum of known inducers of erythroid differentiation in Fried erythroleukemia cells. Among these inducers were: 1) polar solvents such as dimethyl-sulfoxide, dimethylformamide, and hexamethylene bisacetamide; 2) purines such as hypoxanthine, inosine, and adenosine; 3) low-molecular-weight fatty acids such as n-butyrate; and 4) conditions expected to elevate levels of cyclic AMP. In the latter group were activators of adenylate cyclase such as cholera toxin and prostaglandin E 1; cyclic AMP phosphodiesterase inhibitors such as theophylline and 1-methyl-3-isobutylxanthine; and analogs of cyclic AMP. Induction of domes occurred 15--30 h after addition of inducer to the culture medium. Induction by chemicals was serum-dependent and required protein synthesis but not DNA synthesis. Induced dome formation was reversible after removal of inducer, requiring the continuous presence of inducer. Reversal was also observed after either either removal of serum or addition of inhibitors of protein synthesis. These results suggest that hypothesis that domes arise in these epithelial cultures by a process that is similar to cell differentiation and is influenced by cyclic AMP.
J E Lever
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of supramolecular structure     Volume:  12     ISSN:  0091-7419     ISO Abbreviation:  J. Supramol. Struct.     Publication Date:  1979  
Date Detail:
Created Date:  1980-07-22     Completed Date:  1980-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0330464     Medline TA:  J Supramol Struct     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  259-72     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation*
Cell Line
Cell Membrane / drug effects,  ultrastructure*
Cryoprotective Agents / pharmacology
Cyclic AMP / pharmacology
Epithelium / physiology
Mammary Neoplasms, Experimental
Purines / pharmacology
Reg. No./Substance:
0/Cryoprotective Agents; 0/Purines; 0/Solvents; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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