Document Detail


Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron.
MedLine Citation:
PMID:  15792797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.
Authors:
Deborah M Johnson; Sachie Yamaji; Jason Tennant; Surjit K Srai; Paul A Sharp
Related Documents :
7510987 - Heterogeneity of sulfated microdomains within basement membranes of pulmonary airway ep...
9914507 - Preferential activation of iron regulatory protein-2 in cell lines as a result of highe...
2018567 - Evidence that desferrioxamine cannot enter cells by passive diffusion.
16377587 - Cell permeable iron chelators as potential cancer chemotherapeutic agents.
16595717 - Centrosome changes during meiosis in horse oocytes and first embryonic cell cycle organ...
17341627 - Retinoic acid and histone deacetylase inhibitor bml-210 inhibit proliferation of human ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  579     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-28     Completed Date:  2005-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1923-9     Citation Subset:  IM    
Affiliation:
School of Biomedical and Molecular Sciences, University of Surrey, Guildford, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biotinylation
Caco-2 Cells
Cation Transport Proteins / analysis,  genetics,  metabolism*
Cell Membrane / chemistry,  metabolism
Endosomes / chemistry,  metabolism
Humans
Intestinal Mucosa / drug effects,  metabolism*,  physiology
Iron / metabolism,  pharmacology*
Iron-Binding Proteins / analysis,  genetics,  metabolism*
Lysosome-Associated Membrane Glycoproteins
Lysosomes / chemistry,  metabolism
Membrane Glycoproteins / analysis,  metabolism
Chemical
Reg. No./Substance:
0/Cation Transport Proteins; 0/Iron-Binding Proteins; 0/LAMP1 protein, human; 0/Lysosome-Associated Membrane Glycoproteins; 0/Membrane Glycoproteins; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation and structural characterization of epilancin 15X, a novel lantibiotic from a clinical stra...
Next Document:  Mitochondrial localization of Arabidopsis thaliana Isu Fe-S scaffold proteins.