Document Detail

Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron.
MedLine Citation:
PMID:  15792797     Owner:  NLM     Status:  MEDLINE    
A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.
Deborah M Johnson; Sachie Yamaji; Jason Tennant; Surjit K Srai; Paul A Sharp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  579     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-28     Completed Date:  2005-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1923-9     Citation Subset:  IM    
School of Biomedical and Molecular Sciences, University of Surrey, Guildford, UK.
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MeSH Terms
Caco-2 Cells
Cation Transport Proteins / analysis,  genetics,  metabolism*
Cell Membrane / chemistry,  metabolism
Endosomes / chemistry,  metabolism
Intestinal Mucosa / drug effects,  metabolism*,  physiology
Iron / metabolism,  pharmacology*
Iron-Binding Proteins / analysis,  genetics,  metabolism*
Lysosome-Associated Membrane Glycoproteins
Lysosomes / chemistry,  metabolism
Membrane Glycoproteins / analysis,  metabolism
Reg. No./Substance:
0/Cation Transport Proteins; 0/Iron-Binding Proteins; 0/LAMP1 protein, human; 0/Lysosome-Associated Membrane Glycoproteins; 0/Membrane Glycoproteins; 0/solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2; 7439-89-6/Iron

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