Document Detail

Regulation of dietary fatty acid entrapment in subcutaneous adipose tissue and skeletal muscle.
MedLine Citation:
PMID:  12196459     Owner:  NLM     Status:  MEDLINE    
Using stable isotopic labeling of dietary fatty acids in conjunction with arteriovenous difference measurements, we have assessed the regulation of lipoprotein lipase-derived fatty acid entrapment in subcutaneous adipose tissue and forearm muscle in healthy subjects in the postprandial state. Eight volunteers fasted overnight and were then given a mixed meal containing [ 1-(13)C]palmitic acid and [1-(13)C]oleic acid. At baseline and for 6 h after the meal, blood samples were obtained from an arterialized hand vein and veins draining subcutaneous abdominal adipose tissue and forearm muscle, and arteriovenous differences were calculated. Entrapment of labeled fatty acids released by circulating triacylglycerol hydrolysis was close to 100% at 60 min, decreasing to 10-30% by 360 min. Entrapment of labeled fatty acids in forearm muscle was >100% and did not change with time. This study shows that entrapment of dietary fatty acids in adipose tissue in the postprandial period is a highly regulated process (varying with time) and that this can be studied in humans using stable isotope- labeled fatty acids in combination with measurement of appropriate arteriovenous differences. Also, fatty acid trapping in skeletal muscle is fundamentally different from that in adipose tissue, in that all the fatty acids released by lipoprotein lipase in skeletal muscle are taken up by the tissue.
Kevin Evans; Graham C Burdge; Stephen A Wootton; Mo L Clark; Keith N Frayn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes     Volume:  51     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-28     Completed Date:  2002-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2684-90     Citation Subset:  AIM; IM    
Department of Clinical Chemistry, Staffordshire General Hospital, Stafford, UK.
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MeSH Terms
Adipose Tissue / metabolism*
Blood Glucose / analysis
Fatty Acids / administration & dosage*,  metabolism*
Fatty Acids, Nonesterified / blood
Insulin / blood
Middle Aged
Muscle, Skeletal / metabolism*
Postprandial Period
Triglycerides / blood
Reg. No./Substance:
0/Blood Glucose; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Triglycerides; 11061-68-0/Insulin

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