| Regulation of cytokine responses by seasonality of vitamin D status in healthy individuals. | |
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MedLine Citation:
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PMID: 21323660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The immune modulating capacity of vitamin D(3) is well-recognized. Ultra-violet (UV) exposure determines production of vitamin D(3) in vivo and varies through the course of the year, especially in temperate regions. However, it is not known whether the human innate immune response differs due to seasonality. To validate the seasonal effects of vitamin D(3) , the effect of 1,25(OH)(2) D(3) on peripheral blood mononuclear cells (PBMC) cytokine response was first determined in vitro. 1,25(OH)(2) D(3) decreased interleukin (IL)-6 and tumour necrosis factor (TNF)-α release by PBMC stimulated with tripalmitoyl-S-glycerylcysteine (Pam3Cys) or lipopolysaccharide (LPS). Subsequently, ex-vivo stimulation studies were performed in 15 healthy volunteers through the course of the four seasons of the year. PBMC were isolated and stimulated with Toll-like receptor (TLR)-2 and TLR-4 ligands Pam3Cys and LPS, respectively. Circulating concentrations of 25(OH)D(3) and 1,25(OH)(2) D(3) were higher during summer (P<0·05) and a down-regulation of TLR-4-mediated IL-1β, IL-6, TNF-α, interferon (IFN)-γ and IL-10 production in summer was observed compared to winter (P<0·05). The variation in cytokine response upon TLR-2 (Pam3Cys) stimulation was moderate throughout the four seasons. The repressed cytokine production during the summer months could be explained partly by the reduced cell-membrane expression of TLRs. Physiological variation in vitamin D(3) status through the four seasons of the year can lead to alteration in the innate immune responses. Elevated vitamin D(3) level in vivo is associated with down-regulation of cytokine response through diminished surface expression of pattern recognition receptors. |
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Authors:
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A-L Khoo; L Y A Chai; H J P M Koenen; F C G J Sweep; I Joosten; M G Netea; A J A M van der Ven |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-02-16 |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 164 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-11 Completed Date: 2011-06-14 Revised Date: 2012-04-02 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 72-9 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology. |
Affiliation:
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Department of Laboratory Medicine, Laboratory of Medical Immunology, Radboud University Nijmegen, the Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Calcitriol / blood*, pharmacology Cysteine / analogs & derivatives, pharmacology Cytokines / analysis, blood* Down-Regulation Enzyme-Linked Immunosorbent Assay Flow Cytometry Humans Interferon-gamma / analysis, blood Interleukin-1beta / analysis, blood Interleukin-6 / analysis, blood Leukocytes, Mononuclear / cytology, drug effects, metabolism* Lipopolysaccharides / pharmacology Male Middle Aged Seasons* Toll-Like Receptor 2 / metabolism Toll-Like Receptor 4 / metabolism Tumor Necrosis Factor-alpha / analysis, blood Vitamins / blood, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Interleukin-1beta; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 0/Tumor Necrosis Factor-alpha; 0/Vitamins; 32222-06-3/Calcitriol; 52-90-4/Cysteine; 82115-62-6/Interferon-gamma; 87079-94-5/tripalmitoyl cysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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