| Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1. | |
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MedLine Citation:
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PMID: 22395741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The characterization of mesenchymal progenitors is central to understanding development, postnatal pathology and evolutionary adaptability. The precise identity of the mesenchymal precursors that generate the coronal suture, an important structural boundary in mammalian skull development, remains unclear. We show in mouse that coronal suture progenitors originate from hedgehog-responsive cephalic paraxial mesoderm (Mes) cells, which migrate rapidly to a supraorbital domain and establish a unidirectional lineage boundary with neural crest (NeuC) mesenchyme. Lineage tracing reveals clonal and stereotypical expansion of supraorbital mesenchymal cells to form the coronal suture between E11.0 and E13.5. We identify engrailed 1 (En1) as a necessary regulator of cell movement and NeuC/Mes lineage boundary positioning during coronal suture formation. In addition, we provide genetic evidence that En1 functions upstream of fibroblast growth factor receptor 2 (Fgfr2) in regulating early calvarial osteogenic differentiation, and postulate that it plays an additional role in precluding premature osteogenic conversion of the sutural mesenchyme. |
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Authors:
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Ron A Deckelbaum; Greg Holmes; Zhicheng Zhao; Chunxiang Tong; Claudio Basilico; Cynthia A Loomis |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 139 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-03-07 Completed Date: 2012-05-04 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 1346-58 Citation Subset: IM |
Affiliation:
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Department of Pathology, New York University School of Medicine, 550 1st Avenue, New York, NY 10016, USA. ron.deckelbaum@regeneron.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Lineage Cell Movement Crosses, Genetic Female Gene Expression Regulation, Developmental* Homeodomain Proteins / physiology* Male Mesoderm / metabolism* Mice Morphogenesis Neural Crest / cytology* Osteogenesis Skull / embryology Stem Cells / cytology Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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5 P30CA16087-31/CA/NCI NIH HHS; AR051358/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/En1 protein, mouse; 0/Homeodomain Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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