Document Detail


Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1.
MedLine Citation:
PMID:  22395741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The characterization of mesenchymal progenitors is central to understanding development, postnatal pathology and evolutionary adaptability. The precise identity of the mesenchymal precursors that generate the coronal suture, an important structural boundary in mammalian skull development, remains unclear. We show in mouse that coronal suture progenitors originate from hedgehog-responsive cephalic paraxial mesoderm (Mes) cells, which migrate rapidly to a supraorbital domain and establish a unidirectional lineage boundary with neural crest (NeuC) mesenchyme. Lineage tracing reveals clonal and stereotypical expansion of supraorbital mesenchymal cells to form the coronal suture between E11.0 and E13.5. We identify engrailed 1 (En1) as a necessary regulator of cell movement and NeuC/Mes lineage boundary positioning during coronal suture formation. In addition, we provide genetic evidence that En1 functions upstream of fibroblast growth factor receptor 2 (Fgfr2) in regulating early calvarial osteogenic differentiation, and postulate that it plays an additional role in precluding premature osteogenic conversion of the sutural mesenchyme.
Authors:
Ron A Deckelbaum; Greg Holmes; Zhicheng Zhao; Chunxiang Tong; Claudio Basilico; Cynthia A Loomis
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-07     Completed Date:  2012-05-04     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1346-58     Citation Subset:  IM    
Affiliation:
Department of Pathology, New York University School of Medicine, 550 1st Avenue, New York, NY 10016, USA. ron.deckelbaum@regeneron.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Lineage
Cell Movement
Crosses, Genetic
Female
Gene Expression Regulation, Developmental*
Homeodomain Proteins / physiology*
Male
Mesoderm / metabolism*
Mice
Morphogenesis
Neural Crest / cytology*
Osteogenesis
Skull / embryology
Stem Cells / cytology
Time Factors
Grant Support
ID/Acronym/Agency:
5 P30CA16087-31/CA/NCI NIH HHS; AR051358/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/En1 protein, mouse; 0/Homeodomain Proteins
Comments/Corrections

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