Document Detail

Regulation of corneal collagenase production: epithelial-stromal cell interactions.
MedLine Citation:
PMID:  212749     Owner:  NLM     Status:  MEDLINE    
Mixtures of epithelial and stromal cells isolated from normal adult rabbit cornea, when cocultured in the presence of cytochalasin B, produced latent collagenase, whereas neither cell type alone, nor the mixture in the absence of this agent, did so. The enzyme, a characteristic animal collagenase, required proteolytic activation. The relative concentrations of epithelial and stromal cells had a profound effect on on collagenase production, the enzyme activity bieng directly proportional to the number of stromal cells but inversely proportional to the number of epithelial cells. The amount of enzyme released into the medium was also directly proportional to cytochalasin B concentration. Media conditioned by cytochalasin B-treated epithelial or stromal cells did not stimulate collagenase secretion by the other cell type. The data suggest direct cell contact or close proximity as the mode of productive interaction and tentatively identify the stromal cell as the source of enzyme and the epithelial cell as a stimulator.
B Johnson-Muller; J Gross
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  75     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-12-27     Completed Date:  1978-12-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4417-21     Citation Subset:  IM    
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MeSH Terms
Cell Communication
Cornea / cytology,  enzymology*
Cytochalasin B / pharmacology
Dose-Response Relationship, Drug
Epithelium / enzymology
Mesoderm / enzymology
Microbial Collagenase / metabolism*,  secretion
Reg. No./Substance:
14930-96-2/Cytochalasin B; EC Collagenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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