Document Detail

Regulation of connexin43 oligomerization is saturable.
MedLine Citation:
PMID:  16531319     Owner:  NLM     Status:  MEDLINE    
We have used connexin constructs containing a C-terminal di-lysine-based endoplasmic reticulum (ER) retention/retrieval signal (HKKSL) transfected into HeLa cells to study early events in connexin oligomerization. Using this approach, we found that Cx43-HKKSL stably expressed at moderate levels by HeLa cells was retained in the ER and prevented from oligomerization. However, Cx43-HKKSL stably overexpressed by HeLa cells escaped from the ER and localized to a perinuclear region of the cell that included the Golgi apparatus. Overexpressed Cx43-HKKSL oligomerized into hexamers and also formed Triton X-100 insoluble, intracellular complexes that resembled gap junctions. Thus, the ability of HeLa cells to inhibit Cx43 oligomerization was saturable. HeLa cells stably overexpressing Cx43-HKKSL may provide a useful model system to evaluate pharmacologic agents and/or cDNAs encoding chaperones with the potential to regulate initial steps in Cx43 oligomerization.
Jayasri Das Sarma; Shamie Das; Michael Koval
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cell communication & adhesion     Volume:  12     ISSN:  1541-9061     ISO Abbreviation:  Cell Commun. Adhes.     Publication Date:    2005 Jul-Dec
Date Detail:
Created Date:  2006-03-13     Completed Date:  2007-05-31     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  101096596     Medline TA:  Cell Commun Adhes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-47     Citation Subset:  IM    
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MeSH Terms
Cell Communication
Connexin 43 / genetics*,  metabolism
Dipeptides / genetics
Endoplasmic Reticulum / metabolism*
Gap Junctions / genetics,  metabolism
Golgi Apparatus / metabolism*
HeLa Cells
Models, Biological
Protein Sorting Signals / genetics
Recombinant Fusion Proteins / metabolism
Grant Support
GM61012/GM/NIGMS NIH HHS; P01 HL019737/HL/NHLBI NIH HHS; P01 HL019737-26/HL/NHLBI NIH HHS; P01 HL019737-300018/HL/NHLBI NIH HHS; R01 GM061012/GM/NIGMS NIH HHS; R01 GM061012-06/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Connexin 43; 0/Dipeptides; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; 13184-13-9/lysyllysine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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