Document Detail


Regulation of connexin43 expression by c-fos and c-jun in myometrial cells.
MedLine Citation:
PMID:  12064606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Labor is associated with a dramatic increase in the myometrial expression of connexin43 (Cx43) which is thought to mediate myocyte contractile coupling. The transcription factor c-fos is also dramatically increased prior to the onset of labor and is therefore a potential regulator of Cx43. The promoter region of Cx43 contains a conserved activator protein-1 (AP-1) site, which binds dimers of Fos and Jun proteins. We constructed expression vectors for c-Fos and c-Jun to investigate the role of these transcription factors in the regulation of Cx43 expression. These expression vectors were then co-transfected into SHM (syrian hamster myocyte) cells with a Cx43 promoter-Luciferase vector. The combinations of c-Fos and c-Jun proteins activated the Cx43 promoter while c-Jun alone had no effect on Cx43 promoter activity. Mutation of the AP-1 site was found to reduce this responsiveness. These data indicate that the transcription factors c-Fos and c-Jun are important in the regulation of Cx43 expression.
Authors:
J A Mitchell; S J Lye
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell communication & adhesion     Volume:  8     ISSN:  1541-9061     ISO Abbreviation:  Cell Commun. Adhes.     Publication Date:  2001  
Date Detail:
Created Date:  2002-06-14     Completed Date:  2003-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101096596     Medline TA:  Cell Commun Adhes     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  299-302     Citation Subset:  IM    
Affiliation:
Program in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Connexin 43 / genetics*,  metabolism
Cricetinae
Female
Gene Expression Regulation*
Genes, Reporter
Genetic Vectors
Labor, Obstetric / metabolism
Muscle Cells / cytology,  physiology
Myometrium / cytology,  metabolism*
Pregnancy
Promoter Regions, Genetic
Proto-Oncogene Proteins c-fos / genetics,  metabolism*
Proto-Oncogene Proteins c-jun / genetics,  metabolism*
Rats
Transcription Factor AP-1 / metabolism
Chemical
Reg. No./Substance:
0/Connexin 43; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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