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Regulation of connective tissue collagenase production: stimulators from adult and fetal epidermal cells.
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MedLine Citation:
PMID:  6323486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have examined the ability of primary adult rabbit skin cells to regulate collagenase production in vitro. Dermal cells constitutively produce collagenase in culture, and enzyme production by these cells can be influenced by epithelial cells. Co-culture with skin epidermal cells resulted in more enzyme production by dermal cells, whereas co-culture with corneal epithelial cells yielded less enzyme activity. Connective tissue cells from a different source, cornea, also produced collagenase when co-cultured with skin epidermal cells, although the stromal cells alone made no enzyme. The drug cytochalasin B had very little influence on collagenase production by dermal cells, either alone or in co-culture with epidermal cells, but did significantly potentiate enzyme production by corneal stromal cells responding to epidermal effector molecules. Epidermal-cell-conditioned medium from both fetal and adult rabbit skin was a potent source of stimulators (apparent mol wt 20,500 and 55,000) of connective-tissue-cell collagenase production. Stimulator production by epidermal cultures was cell density dependent. Optimal production of stimulators occurred in adult cultures containing 10(6) epidermal cells/ml of medium, and in fetal cultures containing 10(5) cells/ml. Inhibitors of connective tissue cell enzyme production were not detected in conditioned medium from either adult or fetal epidermal cells.
Authors:
B Johnson-Wint; J Gross
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  98     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-05-11     Completed Date:  1984-05-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  90-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Connective Tissue / enzymology*
Epidermis / cytology,  embryology,  enzymology*
Microbial Collagenase / metabolism*
Rabbits
Grant Support
ID/Acronym/Agency:
AM03564/AM/NIADDK NIH HHS; EY02252/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.24.3/Microbial Collagenase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 1 Year: 1984
Volume: 98 Issue: 1
First Page: 90 Last Page: 96
ID: 2113016
Publisher Id: 84162302
PubMed Id: 6323486

Regulation of connective tissue collagenase production: stimulators from adult and fetal epidermal cells


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