| Regulation of collecting duct water permeability independent of cAMP-mediated AVP response. | |
| | |
MedLine Citation:
|
PMID: 1653534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have used the isolated perfused tubule technique, measurements of adenosine 3',5'-cyclic monophosphate (cAMP) content in single tubules, and freeze-fracture electron microscopy to study the basis of high vasopressin-independent (basal) osmotic water permeability (Pf) in the terminal inner medullary collecting duct (IMCD) of the rat. The results confirmed the observation that the basal Pf of the terminal IMCD is considerably higher than that of the initial IMCD. They also showed that the basal Pf of the terminal IMCD is regulated by in vivo factors related to water intake, such that a very high vasopressin-independent Pf can be induced in isolated tubules by prior in vivo thirsting. Tubules from thirsted rats did not display elevated urea permeabilities, nor did they exhibit measurable cAMP levels in the absence of exogenous vasopressin, indicating that the high basal Pf was not due to residual binding of vasopressin to its receptors. Freeze-fracture studies in thirsted rats demonstrated the presence of intramembrane particle (IMP) clusters in both initial and terminal IMCD, with more in the latter. Water loading of the rats suppressed the incidence of clusters almost entirely but did not fully suppress the basal Pf in the terminal IMCD, raising the possibility that a component of transepithelial water transport may occur independently of the vasopressin-regulated IMP clusters. On the basis of these results, we conclude that the vasopressin-independent Pf in the terminal IMCD can be stably elevated to very high levels in response to in vivo thirsting. This elevation appears to be due to a chronic conditioning effect mediated by unknown in vivo factors and is not due to the short-term cAMP-mediated regulatory effect of vasopressin. |
| | |
Authors:
|
S P Lankford; C L Chou; Y Terada; S M Wall; J B Wade; M A Knepper |
Related Documents
:
|
9749824 - Adrenomedullin suppresses atrial natriuretic factor (anf) secretion from isolated atrium. 7364464 - Effect of glucagon on the perfused rat hind-quarter vessles and on perfused coronary ar... 9222564 - Nitric oxide-dependent and -independent vascular hyporeactivity in mesenteric arteries ... 1651704 - The role of the liver in the production of free radicals during halothane anaesthesia i... 10984 - The uptake of choline by rat liver mitochondria. 6166174 - Igg receptors on the mast cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American journal of physiology Volume: 261 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1991 Sep |
Date Detail:
|
Created Date: 1991-10-04 Completed Date: 1991-10-04 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: F554-66 Citation Subset: IM |
Affiliation:
|
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Arginine Vasopressin / pharmacology* Body Water / metabolism Cell Membrane / physiology, ultrastructure Cyclic AMP / physiology* Diuresis / drug effects Freeze Fracturing Kidney Medulla / drug effects, physiology*, ultrastructure Kidney Tubules, Collecting / drug effects, physiology*, ultrastructure Male Mathematics Microscopy, Electron Models, Biological Permeability Rats Rats, Inbred Strains Urea / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
DK-32839/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
113-79-1/Arginine Vasopressin; 57-13-6/Urea; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Low-dose glucocorticoids maintain Na-H exchange in distal colon of adrenalectomized rats.
Next Document: Characterization of positive inotropic effect of endothelin on mammalian ventricular myocardium.