Document Detail

Regulation of cholesterol synthesis in the hyperlipoproteinaemias. Polymorphonuclear leucocyte abnormality specific to familial type II hypercholesterolaemia.
MedLine Citation:
PMID:  208584     Owner:  NLM     Status:  MEDLINE    
A simple procedure has been devised to give virtually pure preparations of polymorphonuclear leucocytes. This has permitted study of the regulation of cholesterol biosynthesis at cell level. Freshly isolated cells from donors with various forms of hyperlipoproteinaemia have been shown to have very low levels of cholesterol synthesis, presumably due to high circulating levels of apoprotein-B in donor plasma [1]. The activity of the rate-limiting enzyme for cholesterol biosynthesis, 3-hydroxy-3-methylglutaryl coenzyme A reductase, rapidly increases as the cells are incubated in lipoprotein-deficient medium, until, by 12 h, cells from patients heterozygous for familial type IIa hypercholesterolaemia are clearly distinguished from other hyperlipoproteinaemias. The possible significance of this finding is discussed in relation to the causation and treatment of atherosclerotic disease.
W F Bremner; J L Third; B Clark; C Corstorphine; T D Lawrie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Atherosclerosis     Volume:  29     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  1978 Mar 
Date Detail:
Created Date:  1978-08-28     Completed Date:  1978-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  291-9     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Cholesterol / biosynthesis*
Cholesterol, Dietary / blood
Chylomicrons / blood
Hydroxymethylglutaryl CoA Reductases / metabolism
Hypercholesterolemia / blood
Hyperlipidemias / blood*,  genetics*
Lipoproteins, LDL / blood
Lipoproteins, VLDL / blood
Neutrophils / metabolism*
Triglycerides / blood
Reg. No./Substance:
0/Cholesterol, Dietary; 0/Chylomicrons; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 0/Triglycerides; 57-88-5/Cholesterol; EC 1.1.1.-/Hydroxymethylglutaryl CoA Reductases

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