Document Detail

Regulation of cholesterol metabolism in adrenal cortex: comparative studies on cholesterol esterase in human adrenal glands.
MedLine Citation:
PMID:  7920899     Owner:  NLM     Status:  MEDLINE    
We have studied the nature and characteristics of cholesterol esterase (CEase) in human adrenal adenoma and hyperplasia tissues showing Cushing's syndrome, comparing with those in normal tissue. Each tissue demonstrated that two pH optima were found at around 4.5 and 8.0. The results of a subcellular distribution study show that acid and alkaline CEase are mainly located in lysosomes and microsomes, respectively. Our previous data suggested that phosphatidylcholine which was sonicated with cholesteryl oleate as a substrate may play a crucial role in the regulation of CEase in rat adrenal. The effect of phosphatidylcholine was therefore investigated in the present study. Acid CEase in normal tissue was increased in a dose-dependent manner by phosphatidylcholine, but not in the adenoma or hyperplasia tissues. None of those tissues showed any enhancement in alkaline CEase activity when phosphatidylcholine was added to the substrates. It is therefore suggested that the mechanism of regulation of CEase among three different kinds of human adrenals may be different from the data for the effect of phosphatidylcholine. Basal activity of acid CEase in adenoma and hyperplasia was significantly higher than that in normal tissue, and also that of alkaline CEase in hyperplasia tissue was significantly higher than that in normal tissue. Thus it is suggested that such an adrenocortical disorder as Cushing's syndrome due to adenoma and diffuse hyperplasia of the adrenal cortex may possess the nature and characteristics of autonomy of steroidogenesis which seems to be induced by the active metabolism of cholesterol, when compared with normal tissue.
T Nishikawa; K Mikami; A Yoshida; M Omura; Y Saito; Y Tamura; S Yoshida
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrine journal     Volume:  40     ISSN:  0918-8959     ISO Abbreviation:  Endocr. J.     Publication Date:  1993 Aug 
Date Detail:
Created Date:  1994-11-18     Completed Date:  1994-11-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9313485     Medline TA:  Endocr J     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  453-9     Citation Subset:  IM    
Department of Medicine, Yokohama Rosai Hospital, Kanagawa, Japan.
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MeSH Terms
Adenoma / metabolism
Adrenal Cortex / drug effects,  metabolism*,  pathology
Adrenal Gland Neoplasms / metabolism
Cholesterol / metabolism*
Hydrogen-Ion Concentration
Middle Aged
Phosphatidylcholines / pharmacology
Sterol Esterase / metabolism*
Subcellular Fractions / enzymology
Reg. No./Substance:
0/Phosphatidylcholines; 57-88-5/Cholesterol; EC Esterase

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