Document Detail


Regulation of chloride self exchange by cAMP in cortical collecting tubule.
MedLine Citation:
PMID:  3014899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hormonal control of Cl transport was examined in rabbit cortical collecting tubules using the lumen-to-bath 36Cl tracer rate coefficient (KCl, nm/s). Tracer movement via Cl-HCO3 exchange was minimized by using HCO3-CO2-free solutions. The electrical driving force was minimized by treating with amiloride. Under these conditions, net Cl transport was zero, yet there was a large KCl that fell 88% on removing bath (trans) Cl. These results are consistent with the mechanism of tracer flux being predominantly Cl self exchange. KCl fell spontaneously with time in vitro; after this decline KCl could be stimulated with 8-bromo-cAMP. cAMP present from the onset of perfusion prevented the time-dependent fall in KCl. When tracer movement was restricted to diffusion by eliminating Cl self exchange (0 Cl bath), cAMP had no effect on KCl. Although both isoproterenol and vasopressin are known to stimulate adenylate cyclase in this epithelium, only isoproterenol mimicked the cAMP effect on KCl. The isoproterenol effect was blocked by either propranolol or prostaglandin E2. Lumen addition of the disulfonic stilbene DIDS had no effect on KCl. Lumen addition of furosemide or trichloromethiazide had minimal or no effect. Taken together, these results indicate that Cl self exchange is regulated by beta-adrenergic agents acting via cAMP. The lack of an effect of vasopressin suggests cellular heterogeneity in this response to cAMP.
Authors:
K Tago; V L Schuster; J B Stokes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  251     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-08-21     Completed Date:  1986-08-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F40-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / analogs & derivatives,  pharmacology
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Amiloride / pharmacology
Animals
Bicarbonates / metabolism
Biological Transport / drug effects
Chlorides / metabolism*
Cyclic AMP / pharmacology*
Dinoprostone
Furosemide / pharmacology
Isoproterenol / pharmacology
Kidney Tubules / drug effects*
Kidney Tubules, Collecting / drug effects*,  metabolism
Mathematics
Propranolol / pharmacology
Prostaglandins E / pharmacology
Rabbits
Time Factors
Grant Support
ID/Acronym/Agency:
AM-01343/AM/NIADDK NIH HHS; AM-25231/AM/NIADDK NIH HHS; HL-14388/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bicarbonates; 0/Chlorides; 0/Prostaglandins E; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 2609-46-3/Amiloride; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 363-24-6/Dinoprostone; 525-66-6/Propranolol; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 54-31-9/Furosemide; 60-92-4/Cyclic AMP; 7683-59-2/Isoproterenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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