| Regulation of chloride secretion in dog tracheal epithelium by protein kinase C. | |
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MedLine Citation:
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PMID: 2447784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of stimulating protein kinase C on Cl- secretion across dog tracheal epithelium were investigated. The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), and the synthetic diacylglycerol, 1-oleolyl-2-acetylglycerol (OAG), which stimulate protein kinase C (PKC), both stimulated short-circuit current (Isc) with Kd of 10 nM and 1 microM, respectively. In Cl(-)-free solution, the increases in Isc were virtually abolished, suggesting that these compounds stimulate Cl- secretion, a hypothesis confirmed for TPA by measurement of 36Cl- fluxes. The stimulations of Cl- secretion were not sensitive to indomethacin, nor were cAMP levels elevated during stimulation. In addition to its transient stimulatory effect, TPA at high doses caused the eventual lowering of the base-line Isc and a block of subsequent stimulation by cAMP-mediated agonists. This was probably not the result of toxicity or an effect on adenylate cyclase or on cAMP-dependent protein kinase. Cell extracts from both cultured and native dog tracheal epithelial cells showed strong PKC activities. These results suggest that PKC may play a role in regulating Cl- secretion across dog tracheal epithelium. |
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Authors:
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R A Barthelson; D B Jacoby; J H Widdicombe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 253 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-01-27 Completed Date: 1988-01-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C802-8 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of California, San Francisco 94143. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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pharmacology Animals Bucladesine / pharmacology Chlorides / secretion* Cyclic AMP / metabolism Diglycerides / pharmacology Dogs Electric Conductivity Epithelium / physiology Histones / metabolism Indomethacin / pharmacology Isoproterenol / pharmacology Phosphorylation Protein Kinase C / physiology* Protein Kinases / metabolism Tetradecanoylphorbol Acetate / pharmacology Trachea / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HL-24136/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Diglycerides; 0/Histones; 16561-29-8/Tetradecanoylphorbol Acetate; 28822-58-4/1-Methyl-3-isobutylxanthine; 362-74-3/Bucladesine; 53-86-1/Indomethacin; 60-92-4/Cyclic AMP; 7683-59-2/Isoproterenol; EC 2.7.-/Protein Kinases; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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