Document Detail

Regulation of cell diameter, For3p localization, and cell symmetry by fission yeast Rho-GAP Rga4p.
MedLine Citation:
PMID:  17377067     Owner:  NLM     Status:  MEDLINE    
Control of cellular dimensions and cell symmetry are critical for development and differentiation. Here we provide evidence that the putative Rho-GAP Rga4p of Schizosaccharomyces pombe controls cellular dimensions. rga4 Delta cells are wider in diameter and shorter in length, whereas Rga4p overexpression leads to reduced diameter of the growing cell tip. Consistent with a negative role in cell growth control, Rga4p protein localizes to the cell sides in a "corset" pattern, and to the nongrowing cell tips. Additionally, rga4 Delta cells show an altered growth pattern similar to that observed in mutants of the formin homology protein For3p. Consistent with these observations, Rga4p is required for normal localization of For3p and for normal distribution of the actin cytoskeleton. We show that different domains of the Rga4p protein mediate diverse morphological functions. The C-terminal GAP domain mediates For3p localization to the cell tips and maintains cell diameter. Conversely, overexpression of the N-terminal LIM homology domain of Rga4p promotes actin cable formation in a For3p-dependent manner. Our studies indicate that Rga4p functionally interacts with For3p and has a novel function in the control of cell diameter and cell growth.
Maitreyi Das; David J Wiley; Saskia Medina; Helen A Vincent; Michelle Larrea; Andrea Oriolo; Fulvia Verde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-03-21
Journal Detail:
Title:  Molecular biology of the cell     Volume:  18     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-25     Completed Date:  2007-10-31     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2090-101     Citation Subset:  IM    
Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33101-1015, USA.
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MeSH Terms
Actins / metabolism
Cell Cycle
Cell Cycle Proteins / genetics,  metabolism*
Cell Shape*
Cytoskeleton / metabolism
GTPase-Activating Proteins / genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Schizosaccharomyces / cytology*,  metabolism
Schizosaccharomyces pombe Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Actins; 0/Cell Cycle Proteins; 0/FOR3 protein, S pombe; 0/GTPase-Activating Proteins; 0/Recombinant Fusion Proteins; 0/Schizosaccharomyces pombe Proteins; 0/rho GTPase-activating protein

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