| Regulation of the cell cycle in response to inhibition of mitochondrial generated energy. | |
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MedLine Citation:
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PMID: 15925326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle control is regulated through the temporal action of both cyclin-dependent kinases and cyclin binding partners. Previously, we have demonstrated that low doses of oligomycin result in a cell cycle arrest of HL-60 cells in G(1) [S. Sweet, G. Singh, Accumulation of human promyelocytic leukemic (HL-60) cells at two energetic cell cycle checkpoints, Cancer Res. 55 (1995) 5164-5167]. In this study, we provide the molecular mechanisms for the observed G(1) arrest following mitochondrial ATPase inhibition. Protein expression of cyclin E and CDK2, the kinase activity of complexed cyclin E/CDK2, and protein expression of p16, p21, and p27 were all unaffected by oligomycin administration. While CDK4 levels were unchanged following oligomycin treatment, a dramatic reduction in cyclin D(1) was observed. Moreover, increased amounts of hypo-phosphorylated retinoblastoma protein (Rbp) and Rbp bound E2F were observed following mitochondrial ATP synthase inhibition. These data provide further evidence that surveillance of available energy occurs during G(1) and ATP deprivation results in cell cycle arrest via a reduction in cyclin D. |
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Authors:
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Adam Gemin; Susan Sweet; Tom J Preston; Gurmit Singh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 332 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-13 Completed Date: 2005-08-04 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1122-32 Citation Subset: IM |
Affiliation:
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Juravinski Cancer Centre, 699 Concession St., Hamilton, Ont., Canada L8V 5C2. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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chemistry Blotting, Western CDC2-CDC28 Kinases / biosynthesis Cell Cycle Cell Cycle Proteins / biosynthesis, metabolism Centrifugation Cyclin D Cyclin D1 / metabolism Cyclin E / biosynthesis Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinase Inhibitor p16 / biosynthesis Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinases / metabolism Cyclins / metabolism DNA / chemistry DNA-Binding Proteins / metabolism E2F Transcription Factors G1 Phase HL-60 Cells Humans Immunoprecipitation Mitochondria / metabolism* Mitochondrial Proton-Translocating ATPases / metabolism Models, Biological Oligomycins / chemistry Phosphorylation Retinoblastoma Protein / metabolism S Phase Transcription Factors / metabolism Tumor Suppressor Proteins / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin D; 0/Cyclin E; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/DNA-Binding Proteins; 0/E2F Transcription Factors; 0/Oligomycins; 0/Retinoblastoma Protein; 0/Transcription Factors; 0/Tumor Suppressor Proteins; 136601-57-5/Cyclin D1; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 56-65-5/Adenosine Triphosphate; 9007-49-2/DNA; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases; EC 3.6.3.-/Mitochondrial Proton-Translocating ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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