| Regulation of cell adhesion signaling by synthetic glycopolymer matrix in primary cultured hepatocyte. | |
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MedLine Citation:
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PMID: 14572665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Control of cell-matrix interactions is a central principle for the design of biomaterial in tissue engineering. In this study, we evaluated a synthetic glycopolymer, which is recognized by the asialoglycoprotein receptor (ASGPR) expressed on the surface of hepatocytes, as an artificial matrix to regulate integrin-mediated signaling. The phosphorylation of focal adhesion kinase was restricted in hepatocytes cultured on the glycopolymer compared with fibronectin. In addition, there was no reorganization of cytoskeleton-related proteins such as actin filaments, microtubules, and vinculin in hepatocytes cultured on the glycopolymer. DNA synthesis and cyclin D1 expression were suppressed in hepatocytes grown on the glycopolymer as compared with those grown on fibronectin and collagen. The data suggest that the glycopolymer will be a good artificial matrix to regulate integrin-mediated signaling and cell growth through the unique ASGPR-carbohydrate interaction. |
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Authors:
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Sang-Heon Kim; Jong-Hun Kim; Toshihiro Akaike |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 553 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-10-23 Completed Date: 2003-12-09 Revised Date: 2012-06-05 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 433-9 Citation Subset: IM |
Affiliation:
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Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501 Yokohama, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Asialoglycoprotein Receptor / metabolism Cell Adhesion / drug effects, physiology* Cell Cycle / drug effects, physiology Collagen / pharmacology Cyclin D1 / biosynthesis, drug effects Cytoskeletal Proteins / analysis, metabolism DNA / biosynthesis, drug effects Extracellular Matrix / metabolism Fibronectins / pharmacology Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Focal Adhesions / drug effects, metabolism Hepatocytes / cytology, drug effects, metabolism, physiology* Integrins / antagonists & inhibitors, metabolism Male Mice Mice, Inbred ICR Phosphorylation Polystyrenes / chemistry, pharmacology* Protein-Tyrosine Kinases / metabolism Signal Transduction / physiology |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoprotein Receptor; 0/Cytoskeletal Proteins; 0/Fibronectins; 0/Integrins; 0/Polystyrenes; 136601-57-5/Cyclin D1; 9007-34-5/Collagen; 9007-49-2/DNA; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/Focal Adhesion Protein-Tyrosine Kinases; EC 2.7.10.2/Ptk2 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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