Document Detail

Regulation of cardiac myosin synthesis: studies of RNA content in cultured heart cells.
MedLine Citation:
PMID:  3678378     Owner:  NLM     Status:  MEDLINE    
Contraction regulates the myosin content and the rate of myosin synthesis in cultured neonatal rat heart cells. To further explore the mechanism for this regulation we examined various parameters of RNA content and RNA synthesis in contacting versus noncontracting myocytes. While contraction stimulated myosin heavy chain (MHC) synthesis by 72% compared to that of KCl-arrested cells, simultaneous analyses of polysome profiles were no different under the two culture conditions. Incorporation of [3H]uridine monophosphate into cellular RNA revealed no change in the rate of total RNA or ribosomal subunits synthesis. In vitro translation of cellular RNA yielded similar incorporation of [35S]methionine into trichloroacetic acid precipitable protein. Specific transcription of the MHC gene was examined by dot-blot analysis and was unaltered by contraction. Northern blot analysis of the MHC sequences detected by a cDNA probe revealed an mRNA sequence corresponding to a molecular weight of approximately 30 S. These data suggest that RNA synthesis and RNA content are unaltered by contraction in cultured heart cells and therefore the changes in myosin synthesis may be mediated at a post-transcriptional control level.
P McDermott; P Whitaker-Dowling; I Klein
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  173     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1987 Nov 
Date Detail:
Created Date:  1987-12-29     Completed Date:  1987-12-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  183-92     Citation Subset:  IM    
University of Pittsburgh School of Medicine, Pennsylvania 15260.
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MeSH Terms
Cells, Cultured
Gene Expression Regulation
Myocardial Contraction
Myocardium / metabolism*
Myosins / biosynthesis*
Polyribosomes / physiology
RNA, Messenger / genetics
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/RNA, Messenger; EC

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