Document Detail


Regulation of carcinoembryonic antigen release from colorectal cancer cells.
MedLine Citation:
PMID:  21725636     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Clinical and experimental evidence suggest that circulating carcinoembryonic antigen (CEA) released from tumor cells has an instrumental role in colorectal cancer-liver metastasis. However, the precise mechanism of the regulation of the CEA release from cancer cells is not known. We investigated if the rate of CEA and another GPI-anchored protein, alkaline phosphatase (AP) release is correlated with cellular glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) expression. We also evaluated the effects of phosphatidic acid (PA), a compound known to inhibit GPI-PLD activity, on the CEA and AP release from colon cancer cells. The expression of CEA, GPI-PLD, and AP in five colon carcinoma cells (LS180, Caco2, SW742, SW1116, and HT29/219) was verified by immunoblot and real-time RT-PCR analysis. The amounts of CEA and AP released into cell culture media were determined using ELISA and a colorimetric assay, respectively. We examined the effects of PA (20-100 μM) on CEA and AP release from LS180 cells. All five cancer cell lines analyzed expressed GPI-PLD protein. While there was a positive relationship between AP release and the levels of GPI-PLD transcript expression, we found no direct correlation between CEA released from cancer cells and the GPI-PLD mRNA expression level. However, the rate of CEA release was positively associated with the level of CEA transcript expression. In comparison to controls, the release of GPI-anchored CEA and AP, but not CA19-9 was inhibited significantly by both crude and pure phosphatidic acid (by 56 and 54.5%, respectively). Using PA for inhibiting CEA release from cancer cells may have therapeutic application in preventing CRC-liver metastasis.
Authors:
Abbas Pakdel; Fakhraddin Naghibalhossaini; Pooneh Mokarram; Mansooreh Jaberipour; Ahmad Hosseini
Related Documents :
1596476 - In vitro contraction rate of collagen in sponge-shape matrices.
22110206 - Detection of tfpi2 methylation in the serum of gastric cancer patients.
22054246 - Tofsims-p: a web-based platform for analysis of large-scale tof-sims data.
22076196 - Prevalence, pathophysiology, screening and management of osteoporosis in gastric cancer...
25048556 - Pdt for barrett's esophagus: status and unsolved problems.
15870976 - A high peripheral microvessel density count correlates with a poor prognosis in pancrea...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-2
Journal Detail:
Title:  Molecular biology reports     Volume:  -     ISSN:  1573-4978     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Zand Street, Shiraz, Fars, Iran.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Flurbiprofen axetil enhances analgesic effect of fentanyl associated with increase in ?-endorphin le...
Next Document:  Characterization of PROFILIN genes from allotetraploid (Gossypium hirsutum) cotton and its diploid p...