Document Detail


Regulation by thyroid hormone of the expression of basement membrane components in rat prepubertal Sertoli cells.
MedLine Citation:
PMID:  9449648     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study reports the modulation of basement membrane (BM) components, laminin, entactin, and type IV collagen, expression in prepubertal rat Sertoli cell by the thyroid hormone T3. Immunocytochemical studies of permeabilized Sertoli cells in culture showed that T3 treatment (10[-7] M for 24 h) increased the number of cells staining positive for laminin and/or entactin (from 58 +/- 5.3% to 86.4 +/- 6.5%, P < 0.01). In contrast, a strong inhibition of type IV collagen immunopositivity was observed. Western blot analysis of Sertoli cell-conditioned media indicated that T3 treatment significantly (P < 0.01) increased the level of secreted entactin by 60-65% without affecting the levels of laminin A and B1/B2 chains. Moreover, thyroid hormone treatment of Sertoli cells significantly reduced type IV collagen secretion by 62% (P < 0.05). Slot blot analysis of poly-A RNA demonstrated a significant (P < 0.01) increase in the level of entactin messenger RNA (mRNA) by 140% (P < 0.01) and a 50% reduction of type IV collagen alpha1 chain mRNA after thyroid hormone treatment. No effect of the hormone was observed on the accumulation of the laminin B1 and B2 chain mRNAs in Sertoli cell cultures. These effects cannot be ascribed to changes in the degradation of BM components, because no effect of thyroid hormone was observed on plasminogen activators or metalloproteinase secretion by Sertoli cells. These observations indicate the Sertoli cell as a source of entactin within the testis, demonstrate the ability of T3 to differentially regulate the expression of BM components, and can be regarded as a part of the integrated mechanism by which thyroid hormone affects testicular development and differentiation.
Authors:
S Ulisse; N Rucci; D Piersanti; E Carosa; F M Graziano; A Pavan; P Ceddia; M Arizzi; P Muzi; L Cironi; L Gnessi; M D'Armiento; E A Jannini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  139     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-02-18     Completed Date:  1998-02-18     Revised Date:  2010-04-12    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  741-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Experimental Medicine, University of L'Aquila, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basement Membrane / metabolism
Collagen / genetics,  metabolism
Extracellular Matrix / metabolism
Laminin / genetics,  metabolism
Male
Membrane Glycoproteins / genetics,  metabolism
RNA, Messenger / metabolism
Rats
Rats, Wistar
Sertoli Cells / drug effects*,  enzymology,  metabolism*
Triiodothyronine / pharmacology*
Chemical
Reg. No./Substance:
0/Laminin; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/nidogen; 6893-02-3/Triiodothyronine; 9007-34-5/Collagen

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