Document Detail

Regulation by intestinal γδ T cells during establishment of food allergic sensitization in mice.
MedLine Citation:
PMID:  20880210     Owner:  NLM     Status:  In-Data-Review    
To cite this article: Bol-Schoenmakers M, Marcondes Rezende M, Bleumink R, Boon L, Man S, Hassing I, Fiechter D, Pieters RHH, Smit JJ. Regulation by intestinal γδ T cells during establishment of food allergic sensitization in mice. Allergy 2011; 66: 331-340. ABSTRACT: Background:  Food allergy affects approximately 5% of children and is the leading cause of hospitalization for anaphylactic reactions in westernized countries. The mucosal adjuvant cholera toxin induces allergic sensitization to co-administered proteins in mice, while feeding the protein alone induces oral tolerance. Intestinal γδ T cells could be of importance in the induction of oral tolerance. This study aims to investigate whether γδ T cells have functional relevance in food allergic sensitization. Methods:  Changes in γδ T cells on days 1, 2, 3, and 7 after initiation of food allergy were evaluated using flowcytometry. Furthermore, the anti-γδ T-cell receptor (TCR) antibody UC7 was used to block the γδ TCR in mice in vivo, followed by sensitization to peanut. After 4 weeks, peanut-specific antibodies in serum and cytokine production in spleen were measured. Results:  Induction of food allergy resulted in a profound decrease in the percentage of γδ T cells in intestinal tissues and Peyer's Patches, but not in mesenteric lymph nodes or spleen. This decrease could be detected from days 1 to 2 after the initiation of food allergy and the number of γδ T cells returned to normal on day 7. Blockade of the γδ TCR resulted in elevated food allergic responses upon sensitization with peanut characterized by increased IgE and Th2 cytokine production in splenocytes. Conclusion:  These results demonstrate a unique regulatory role of γδ T cells, suggesting that targeting γδ T cells in the intestine may contribute to strategies to prevent and possibly treat food allergy.
M Bol-Schoenmakers; M Marcondes Rezende; R Bleumink; L Boon; S Man; I Hassing; D Fiechter; R H H Pieters; J J Smit
Publication Detail:
Type:  Journal Article     Date:  2010-09-29
Journal Detail:
Title:  Allergy     Volume:  66     ISSN:  1398-9995     ISO Abbreviation:  Allergy     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  331-40     Citation Subset:  IM    
Copyright Information:
© 2010 John Wiley & Sons A/S.
Institute for Risk Assessment Sciences, Utrecht University, Utrecht Utrecht Center for Food Allergy, Utrecht Bioceros BV, Utrecht, the Netherlands.
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