Document Detail

Regulation by estrogen of synthesis and secretion of apolipoprotein A-I in the chicken hepatoma cell line, LMH-2A.
MedLine Citation:
PMID:  12788226     Owner:  NLM     Status:  MEDLINE    
The synthesis and secretion of apolipoprotein A-I (apoA-I) in response to the treatment with estrogen were investigated in the chicken hepatoma cell line, LMH-2A. Exposure of these cells to exogenous estrogen for up to 48 h results in a decrease of apoA-I production, as evident from Western blotting, immunoprecipitation, and immunofluorescence experiments. Likewise, the secretion of apoA-I is also decreased in estrogen-treated cells when compared to controls. However, under both conditions, the disappearance of the apoprotein from the cells occurs very rapidly and with similar kinetics. The bulk of apoA-I secreted from LMH-2A cells is recovered on lipoprotein particles with a buoyant density of > or =1.10 g/ml, corresponding to HDL and heavy LDL. Interestingly, apoA-I is detectable on apoB-containing lipoproteins by sequential immunoprecipitation, suggesting that the two apoproteins co-reside at least on a subfraction of the secreted particles, or that apoB- and apoA-I-containing particles interact. These interactions are more pronounced in estrogen-treated cells, most likely due to the dramatic estrogen-mediated induction of apoB synthesis and secretion.
Marcela Hermann; Roland Foisner; Wolfgang J Schneider; N Erwin Ivessa
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1641     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2003-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Department of Molecular Genetics, Institute of Medical Biochemistry, University and Biocenter Vienna, Dr. Bohr-Gasse 9/2, Vienna A-1030, Austria.
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MeSH Terms
Apolipoprotein A-I / biosynthesis*
Apolipoproteins B / biosynthesis,  blood,  chemistry
Cell Line
Estrogens / pharmacology*
Ethinyl Estradiol / analogs & derivatives,  pharmacology
Gene Expression Regulation, Neoplastic*
Lipoproteins, HDL / blood,  chemistry
Liver Neoplasms, Experimental / metabolism*
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoproteins B; 0/Estrogens; 0/Lipoproteins, HDL; 57-63-6/Ethinyl Estradiol

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