Document Detail

Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus.
MedLine Citation:
PMID:  18541149     Owner:  NLM     Status:  MEDLINE    
The branched-chain alpha-keto acid dehydrogenase (BCKDH) complex is the most important regulatory enzyme in branched-chain amino acid (BCAA) catabolism. We examined the regulation of hepatic BCKDH complex activity in spontaneous type 2 diabetes Otsuka Long-Evans Tokushima Fatty (OLETF) rats and Zucker diabetic fatty rats. Hepatic BCKDH complex activity in these rats was significantly lower than in corresponding control rats. The amount of BCKDH complex in OLETF rats corresponded to the total activity of the complex. Activity and abundance of the bound form of BCKDH kinase, which is responsible for inactivation of the complex, showed an inverse correlation to BCKDH complex activity in OLETF rats. Dietary supplementation of 5% BCAAs for 10 weeks markedly increased BCKDH complex activity, and decreased the activity and bound form of BCKDH kinase in the rats. These results suggest that BCAA catabolism in type 2 diabetes is downregulated and enhanced by BCAA supplementation.
Teiji Kuzuya; Yoshiaki Katano; Isao Nakano; Yoshiki Hirooka; Akihiro Itoh; Masatoshi Ishigami; Kazuhiko Hayashi; Takashi Honda; Hidemi Goto; Yuko Fujita; Rie Shikano; Yuji Muramatsu; Gustavo Bajotto; Tomohiro Tamura; Noriko Tamura; Yoshiharu Shimomura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-09
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  373     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-01     Completed Date:  2008-07-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-8     Citation Subset:  IM    
Department of Gastroenterology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
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MeSH Terms
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) / metabolism*
Amino Acids, Branched-Chain / administration & dosage,  blood,  metabolism*
Citrate (si)-Synthase / metabolism
Diabetes Mellitus, Type 2 / enzymology,  metabolism*
Disease Models, Animal
Liver / enzymology*
Protein Kinases / metabolism*
Rats, Inbred Strains
Reg. No./Substance:
0/Amino Acids, Branched-Chain; EC Dehydrogenase (Lipoamide); EC (si)-Synthase; EC 2.7.-/Protein Kinases; EC 2.7.1.-/BCKAD kinase

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