Document Detail

Regulation of brain glycosylphosphatidylinositol-specific phospholipase D by natural amphiphiles.
MedLine Citation:
PMID:  10591409     Owner:  NLM     Status:  MEDLINE    
Brain glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD)-catalyzed conversion of amphiphilic form of Zn2+ -glycerophosphocholine cholinephosphodiesterase (Amp-GPC PDE) into hydrophilic form was investigated in the presence of natural amphiphiles. Monoacylglycerols enhanced considerably the conversion by GPI-PLD of Amp-GPC PDE to hydrophilic form, with the enhancing effect of monoacylglycerols being dependent on the size of acyl group (C8-C18). Whereas the maximal enhancement of GPI-PLD action was the greatest with monodecanoylglycerol, the concentration (EC50) required to achieve 50% maximal effect was the smallest for monomyristoyl- or monopalmitoylglycerol. In addition, monolaurylglycerol or its alkyl analogue, monododecylglycerol, showed a remarkable decrease in enhancing effect at high concentrations (>1 mM). Presence of double bond in acyl chain, as exemplified by monooleoylglycerol or mono-11-eicosenoin, further enhanced the conversion by GPI-PLD. Meanwhile, lysophosphatidylcholine (IC50, 25 microM) and phosphatidic acid (IC50, >100 microM), ionic amphiphiles, inhibited the GPI-PLD activity, which was determined in the presence of monooleoylglycerol as a detergent. From these results, it is suggested that the activity of GPI-PLD in vivo system may be regulated by natural amphiphiles.
J Y Lee; H J Lee; M R Kim; P K Myung; D E Sok
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  24     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-10     Completed Date:  2000-01-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1577-83     Citation Subset:  IM    
College of Pharmacy, Chungnam National University, Taejon, Korea.
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MeSH Terms
Brain / enzymology*
Glycerides / metabolism*
Phospholipase D / isolation & purification,  metabolism*
Phosphoric Diester Hydrolases / metabolism*
Substrate Specificity
Zinc / metabolism
Reg. No./Substance:
0/Glycerides; 7440-66-6/Zinc; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC cholinephosphodiesterase; EC D; EC phospholipase D

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