| Regulation of brain glycosylphosphatidylinositol-specific phospholipase D by natural amphiphiles. | |
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MedLine Citation:
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PMID: 10591409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brain glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD)-catalyzed conversion of amphiphilic form of Zn2+ -glycerophosphocholine cholinephosphodiesterase (Amp-GPC PDE) into hydrophilic form was investigated in the presence of natural amphiphiles. Monoacylglycerols enhanced considerably the conversion by GPI-PLD of Amp-GPC PDE to hydrophilic form, with the enhancing effect of monoacylglycerols being dependent on the size of acyl group (C8-C18). Whereas the maximal enhancement of GPI-PLD action was the greatest with monodecanoylglycerol, the concentration (EC50) required to achieve 50% maximal effect was the smallest for monomyristoyl- or monopalmitoylglycerol. In addition, monolaurylglycerol or its alkyl analogue, monododecylglycerol, showed a remarkable decrease in enhancing effect at high concentrations (>1 mM). Presence of double bond in acyl chain, as exemplified by monooleoylglycerol or mono-11-eicosenoin, further enhanced the conversion by GPI-PLD. Meanwhile, lysophosphatidylcholine (IC50, 25 microM) and phosphatidic acid (IC50, >100 microM), ionic amphiphiles, inhibited the GPI-PLD activity, which was determined in the presence of monooleoylglycerol as a detergent. From these results, it is suggested that the activity of GPI-PLD in vivo system may be regulated by natural amphiphiles. |
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Authors:
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J Y Lee; H J Lee; M R Kim; P K Myung; D E Sok |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurochemical research Volume: 24 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-01-10 Completed Date: 2000-01-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1577-83 Citation Subset: IM |
Affiliation:
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College of Pharmacy, Chungnam National University, Taejon, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / enzymology* Cattle Glycerides / metabolism* Kinetics Phospholipase D / isolation & purification, metabolism* Phosphoric Diester Hydrolases / metabolism* Substrate Specificity Zinc / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycerides; 7440-66-6/Zinc; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.38/glycerophosphocholine cholinephosphodiesterase; EC 3.1.4.4/Phospholipase D; EC 3.1.4.50/glycoprotein phospholipase D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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