Document Detail

Regulation of the association between PSTPIP and CD2 in murine T cells.
MedLine Citation:
PMID:  11599917     Owner:  NLM     Status:  MEDLINE    
Prominent in T cells and natural killer cells, CD2 binding protein 1 (CD2BP1) plays an important role in CD2-mediated adhesion and signal transduction. In the current study, we investigated CD2 and PSTPIP (proline, serine, threonine phosphatase interacting protein, murine homologue of CD2BP1) interactions in purified mouse splenic T cells. PSTPIP associated with CD2 in both resting and activated T cells. Following various stimuli, such as concanavalin A, anti-TCRbeta, anti-CD3epsilon, anti-CD3epsilon/phorbol myristate acetate (PMA), IL-2, or PMA/ionomycin, PSTPIP and CD2 expression, as well as their association, increased in a time-dependent fashion. While PSTPIP expression and CD2 expression were comparable across most groups, the PSTPIP-CD2 association stimulated by anti-CD3epsilon alone was significantly greater than with other stimuli. Stimulation by anti-CD3epsilon plus anti-CD28 induced even greater PSTPIP-CD2 association than anti-CD3epsilon treatment alone, indicating that CD28 initiated signals are involved in regulating this interaction. There was no direct association between CD3epsilon or CD28 and PSTPIP. Tyrosine phosphorylated PSTPIP bound poorly to CD2 compared to dephosphorylated PSTPIP, and protein tyrosine phosphatase was shown to affect both phosphorylation of PSTPIP and the CD2-PSTPIP association. In addition to CD2, PSTPIP associated with CD4, CD8, CD54, and CD62L. CD2 and CD4 ligation reciprocally regulated their association with PSTPIP. These findings indicate that T cell activation, particularly through the CD3 and CD28 signal transduction pathways, regulates PSTPIP-CD2 interactions. PSTPIP likely has additional broader effects through interactions with CD4, CD8, CD54, and CD62L, and this may influence T cell responses to antigen.
Y Bai; Y Ding; S Spencer; L A Lasky; J S Bromberg
Related Documents :
12138187 - Disruption of mekk2 in mice reveals an unexpected role for mekk2 in modulating t-cell r...
7499277 - Selective regulation of lyn tyrosine kinase by cd45 in immature b cells.
19403707 - Vitamin e reverses impaired linker for activation of t cells activation in t cells from...
8164687 - The cytoplasmic domain of cd28 is both necessary and sufficient for costimulation of in...
19639307 - Differential expression of receptor protein tyrosine phosphatases accompanies the reorg...
9388267 - Nitric oxide inhibits apoptosis by preventing increases in caspase-3-like activity via ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  71     ISSN:  0014-4800     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-15     Completed Date:  2001-12-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  115-24     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, New York, New York 10029-6574, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adaptor Proteins, Signal Transducing*
Antibodies, Monoclonal
Antigens, CD2 / metabolism*
Antigens, CD28 / physiology
Antigens, CD3 / physiology
Blotting, Western
Carrier Proteins / metabolism*
Cytoskeletal Proteins / metabolism*
Lymphocyte Activation*
Mice, Inbred CBA
Mice, Knockout
Precipitin Tests
Protein Tyrosine Phosphatases / pharmacology
Receptors, Antigen, T-Cell / metabolism
T-Lymphocytes / physiology*
Grant Support
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Antibodies, Monoclonal; 0/Antigens, CD2; 0/Antigens, CD28; 0/Antigens, CD3; 0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/Pstpip1 protein, mouse; 0/Receptors, Antigen, T-Cell; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification of TNF-alpha-sensitive sites in HCMVie1 promoter.
Next Document:  Effect of abdominal surgery on the activity of acid and alkaline ribonucleases in rats.