Document Detail


Regulation of aspartate-derived amino-acid metabolism in Zygosaccharomyces rouxii compared to Saccharomyces cerevisiae.
MedLine Citation:
PMID:  10862915     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
To elucidate the growth inhibitory effect of threonine, the regulation of the aspartate-derived amino-acid metabolism in Zygosaccharomyces rouxii, an important yeast for the flavor development in soy sauce, was investigated. It was shown that threonine inhibited the growth of Z. rouxii by blocking the methionine synthesis. It seemed that threonine blocked this synthesis by inhibiting the conversion of aspartate. In addition, it was shown that the growth of Z. rouxii, unlike that of Saccharomyces cerevisiae, was not inhibited by the herbicide sulfometuron methyl (SMM). From enzyme assays, it was concluded that the acetohydroxy acid synthase in Z. rouxii, unlike that in S. cerevisiae, was not sensitive to SMM. Furthermore, the enzyme assays demonstrated that the activity of threonine deaminase in Z. rouxii, like in S. cerevisiae, was strongly inhibited by isoleucine and stimulated by valine. From this work, it is clear that the aspartate-derived amino-acid metabolism in Z. rouxii only partly resembles that in S. cerevisiae.
Authors:
van der Sluis C; Smit; Hartmans; ter Schure EG; Tramper; Wijffels
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  27     ISSN:  0141-0229     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-06-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  151-156     Citation Subset:  -    
Affiliation:
Wageningen University, Department of Agrotechnology and Food Sciences, Food and Bioprocess Engineering Group, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands
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