| Regulation of apoptosis in interleukin-3-dependent hemopoietic cells by interleukin-3 and calcium ionophores. | |
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MedLine Citation:
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PMID: 2118109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An immortalized interleukin-3 (IL-3)-dependent progenitor cell line, BAF-3, undergoes programmed cell death (apoptosis) when deprived of IL-3. This program is characterized by an early degradation of DNA into oligonucleosome-length fragments that precedes by several hours the loss of cell viability. In the absence of IL-3, DNA fragmentation and cell death can be prevented by the calcium ionophores A23187 (1 microM) and ionomycin (0.5 microM). This addition of calcium ionophore maintains cell viability while reversibly arresting the cell cycle. Apoptosis by growth factor deprivation is also a mechanism of cell elimination in bone marrow cells removed from the stromal micro-environment, as DNA fragmentation and cell death was shown to take place in primary cultures of IL-3-responsive bone marrow cells after IL-3 removal. |
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Authors:
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G Rodriguez-Tarduchy; M Collins; A López-Rivas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 9 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1990-10-04 Completed Date: 1990-10-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2997-3002 Citation Subset: IM |
Affiliation:
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Instituto de Investigaciones Biomédicas CSIC, Facultad de Medicina UAM, Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow Cells Calcimycin / pharmacology* Calcium / physiology Cell Cycle / drug effects Cell Survival / drug effects* Cells, Cultured DNA / isolation & purification DNA Replication / drug effects Hematopoietic Stem Cells / cytology*, drug effects, immunology Interleukin-3 / pharmacology* Ionomycin / pharmacology* Mice |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-3; 52665-69-7/Calcimycin; 56092-81-0/Ionomycin; 7440-70-2/Calcium; 9007-49-2/DNA |
| Comments/Corrections | |
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