Document Detail

Regulation of apoptosis in interleukin-3-dependent hemopoietic cells by interleukin-3 and calcium ionophores.
MedLine Citation:
PMID:  2118109     Owner:  NLM     Status:  MEDLINE    
An immortalized interleukin-3 (IL-3)-dependent progenitor cell line, BAF-3, undergoes programmed cell death (apoptosis) when deprived of IL-3. This program is characterized by an early degradation of DNA into oligonucleosome-length fragments that precedes by several hours the loss of cell viability. In the absence of IL-3, DNA fragmentation and cell death can be prevented by the calcium ionophores A23187 (1 microM) and ionomycin (0.5 microM). This addition of calcium ionophore maintains cell viability while reversibly arresting the cell cycle. Apoptosis by growth factor deprivation is also a mechanism of cell elimination in bone marrow cells removed from the stromal micro-environment, as DNA fragmentation and cell death was shown to take place in primary cultures of IL-3-responsive bone marrow cells after IL-3 removal.
G Rodriguez-Tarduchy; M Collins; A López-Rivas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  9     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-10-04     Completed Date:  1990-10-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2997-3002     Citation Subset:  IM    
Instituto de Investigaciones Biomédicas CSIC, Facultad de Medicina UAM, Madrid, Spain.
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MeSH Terms
Bone Marrow Cells
Calcimycin / pharmacology*
Calcium / physiology
Cell Cycle / drug effects
Cell Survival / drug effects*
Cells, Cultured
DNA / isolation & purification
DNA Replication / drug effects
Hematopoietic Stem Cells / cytology*,  drug effects,  immunology
Interleukin-3 / pharmacology*
Ionomycin / pharmacology*
Reg. No./Substance:
0/Interleukin-3; 52665-69-7/Calcimycin; 56092-81-0/Ionomycin; 7440-70-2/Calcium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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