Document Detail


Regulation of apolipoprotein secretion by biliary lipids in newborn swine intestinal epithelial cells.
MedLine Citation:
PMID:  9950808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biliary lipids, composed of bile acids, cholesterol, and phosphatidylcholine, are a major source of luminal lipid in the small intestine. In the present study in a newborn swine intestinal epithelial cell line (IPEC-1), taurocholate and phosphatidylcholine were found to have no effect on apolipoprotein B (apo B) secretion but did significantly increase the basolateral secretion of apo A-I. This regulation of apo A-I secretion occurred at the pretranslational level for taurocholate and at the posttranslational level for phosphatidylcholine. The regulation of apo A-I secretion by phosphatidylcholine did not involve changes in apo A-I degradation and may involve mobilization of a preformed pool of apo A-I. Cholesterol, whether solubilized with taurocholate or phosphatidylcholine, had no effect on the secretion of either apo B or apo A-I. However, when taurocholate, phosphatidylcholine, and cholesterol were combined, apo B secretion was decreased, and the increase in apo A-I secretion noted with taurocholate and phosphatidylcholine alone was ablated. Another primary bile acid, taurochenodeoxycholate, was found to decrease apo B secretion but had no effect on apo A-I secretion. However, the significance of this effect is uncertain, since this bile acid caused significant cellular membrane injury, as evidenced by increased apical medium lactate dehydrogenase activity. Phosphatidylcholine, but not taurocholate, dramatically increased the basolateral secretion of radiolabeled phospholipid with a modest increase in cellular triglyceride radiolabeling. Furthermore, this effect of phosphatidylcholine on lipid synthesis did not require significant hydrolysis or uptake of the phosphatidylcholine molecule. Studies using radiolabeled taurocholate did not demonstrate active transport of taurocholate by these cells.
Authors:
H Wang; R Roberson; J Du; J K Eshun; H M Berschneider; D D Black
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  276     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-30     Completed Date:  1999-03-30     Revised Date:  2011-09-22    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G353-62     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Arkansas Children's Hospital Research Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72202, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / physiology*
Apolipoprotein A-I / genetics,  metabolism
Apolipoproteins / metabolism*
Apolipoproteins B / metabolism
Bile / metabolism*
Cell Line
Cholesterol / pharmacology
Intestinal Mucosa / cytology,  metabolism*
Lipids / physiology*
Phosphatidylcholines / pharmacokinetics,  pharmacology
RNA, Messenger / metabolism
Swine
Taurocholic Acid / pharmacokinetics,  pharmacology
Triglycerides / metabolism
Grant Support
ID/Acronym/Agency:
DK-42100/DK/NIDDK NIH HHS; HD-22551/HD/NICHD NIH HHS; R01 HD022551-08/HD/NICHD NIH HHS; R01 HD022551-09A1/HD/NICHD NIH HHS; R01 HD022551-11/HD/NICHD NIH HHS; R01 HD022551-12/HD/NICHD NIH HHS; R01 HD022551-13/HD/NICHD NIH HHS; R01 HD022551-14A1/HD/NICHD NIH HHS; R01 HD022551-15/HD/NICHD NIH HHS; R01 HD022551-16/HD/NICHD NIH HHS; R01 HD022551-17/HD/NICHD NIH HHS; R01 HD022551-18/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoproteins; 0/Apolipoproteins B; 0/Lipids; 0/Phosphatidylcholines; 0/RNA, Messenger; 0/Triglycerides; 57-88-5/Cholesterol; 81-24-3/Taurocholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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