Document Detail

Regulation of angiotensin type 2 receptor in bovine granulosa cells.
MedLine Citation:
PMID:  18583424     Owner:  NLM     Status:  MEDLINE    
Angiotensin II (AngII) is best known for its role in blood pressure regulation, but it also has documented actions in the reproductive system. There are two AngII receptors, type 1 (AGTR1) and type 2 (AGTR2). AGTR2 mediates the noncardiovascular effects of AngII and is expressed in the granulosa cell layer in rodents and is associated with follicle atresia. In contrast, expression of AGTR2 is reported to occur only in theca cells in cattle. The objective of the present study was to determine whether AngII also plays a role in follicle atresia in cattle. RT-PCR demonstrated AGTR2 mRNA in both granulosa and theca cells of bovine follicles. The presence of AGTR2 protein was confirmed by immunofluorescence. Abundance of AGTR2 mRNA in granulosa cells was higher in healthy compared with atretic follicles, whereas in theca cells, it did not change. Granulosa cells were cultured in serum-free medium, and treatment with hormones that increase estradiol secretion (FSH, IGF-I, and bone morphogenetic protein-7) increased AGTR2 mRNA and protein levels, whereas fibroblast growth factors inhibited estradiol secretion and AGTR2 protein levels. The addition of AngII or an AGTR2-specific agonist to granulosa cells in culture did not affect estradiol secretion or cell proliferation but inhibited abundance of mRNA encoding serine protease inhibitor E2, a protein involved in tissue remodeling. Because estradiol secretion is a major marker of nonatretic granulosa cells, these data suggest that AngII is not associated with follicle atresia in cattle but may have other specific roles during follicle growth.
Valério M Portela; Paulo B D Gonçalves; Angela M Veiga; Edmir Nicola; José Buratini; Christopher A Price
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-26
Journal Detail:
Title:  Endocrinology     Volume:  149     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-23     Completed Date:  2008-11-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5004-11     Citation Subset:  AIM; IM    
Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
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MeSH Terms
Angiotensin II / pharmacology
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins / pharmacology
Cells, Cultured
Estradiol / metabolism,  secretion
Fibroblast Growth Factors / pharmacology
Follicle Stimulating Hormone / pharmacology
Follicular Atresia / physiology*
Granulosa Cells / cytology,  drug effects,  physiology*
Insulin-Like Growth Factor I / pharmacology
RNA, Messenger / metabolism
Receptor, Angiotensin, Type 1 / genetics,  metabolism
Receptor, Angiotensin, Type 2 / genetics*,  metabolism*
Theca Cells / cytology,  physiology
Transforming Growth Factor beta / pharmacology
Vasoconstrictor Agents / pharmacology
Reg. No./Substance:
0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/Receptor, Angiotensin, Type 2; 0/Transforming Growth Factor beta; 0/Vasoconstrictor Agents; 11128-99-7/Angiotensin II; 50-28-2/Estradiol; 62031-54-3/Fibroblast Growth Factors; 67763-96-6/Insulin-Like Growth Factor I; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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