| Regulation of adrenal aldosterone production by serine protease prostasin. | |
| | |
MedLine Citation:
|
PMID: 20204133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A serine protease prostasin has been demonstrated to have a pivotal role in the activation of the epithelial sodium channel. Systemic administration of adenovirus carrying human prostasin gene in rats resulted in an increase in plasma prostasin and aldosterone levels. However, the mechanism by which the elevation of prostasin levels in the systemic circulation stimulated the plasma aldosterone levels remains unknown. Therefore, we examined if prostasin increases the aldosterone synthesis in a human adrenocortical cell line (H295R cells). Luciferase assay using CYP11B2 promoter revealed that prostasin significantly increased the transcriptional activity of CYP11B2. Prostasin significantly increased both CYP11B2 mRNA expression and aldosterone production in a dose-dependent manner. Surprisingly, treatment with camostat mesilate, a potent prostasin inhibitor, had no effect on the aldosterone synthesis by prostasin and also a protease-dead mutant of prostasin significantly stimulated the aldosterone production. A T-type/L-type calcium channel blocker and a protein kinase C (PKC) inhibitor significantly reduced the aldosterone synthesis by prostasin. Our findings suggest a stimulatory effect of prostasin on the aldosterone synthesis by adrenal gland through the nonproteolytic action and indicate a new role of prostasin in the systemic circulation. |
| | |
Authors:
|
Takehiro Ko; Yutaka Kakizoe; Naoki Wakida; Manabu Hayata; Kohei Uchimura; Naoki Shiraishi; Taku Miyoshi; Masataka Adachi; Shizuka Aritomi; Tomoyuki Konda; Kimio Tomita; Kenichiro Kitamura |
Related Documents
:
|
15805423 - Altered expression patterns of heterogeneous nuclear ribonucleoproteins a2 and b1 in th... 22685153 - Development and application of an arabinose-inducible expression system by facilitating... 21949733 - Glatiramer acetate treatment normalizes deregulated microrna expression in relapsing re... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-02 |
Journal Detail:
|
Title: Journal of biomedicine & biotechnology Volume: 2010 ISSN: 1110-7251 ISO Abbreviation: J. Biomed. Biotechnol. Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-03-05 Completed Date: 2010-05-25 Revised Date: 2010-09-28 |
Medline Journal Info:
|
Nlm Unique ID: 101135740 Medline TA: J Biomed Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 793843 Citation Subset: IM |
Affiliation:
|
Department of Nephrology, Kumamoto University Graduate School of Medical Sciences, 1-1-1 Honjo, Kumamoto, Kumamoto 860-8556, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Cortex
/
drug effects,
enzymology,
metabolism* Aldosterone / biosynthesis*, genetics Aldosterone Synthase / biosynthesis, genetics, metabolism Angiotensin II Type 1 Receptor Blockers / pharmacology Animals Calcium Channel Blockers / pharmacology Cell Line Dihydropyridines / pharmacology Gene Expression Regulation Gene Silencing Humans Nitrophenols / pharmacology Organophosphorus Compounds / pharmacology Promoter Regions, Genetic Rats Receptors, Proteinase-Activated / genetics, metabolism Recombinant Proteins / pharmacology Serine Endopeptidases / genetics, metabolism*, pharmacology Tetrazoles / pharmacology Transfection Valine / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Angiotensin II Type 1 Receptor Blockers; 0/Calcium Channel Blockers; 0/Dihydropyridines; 0/Nitrophenols; 0/Organophosphorus Compounds; 0/Receptors, Proteinase-Activated; 0/Recombinant Proteins; 0/Tetrazoles; 111011-53-1/efonidipine; 137862-53-4/valsartan; 52-39-1/Aldosterone; 7004-03-7/Valine; EC 1.14.15.4/Aldosterone Synthase; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.-/prostasin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Massive fetomaternal hemorrhage caused by an intraplacental choriocarcinoma: a case report.
Next Document: Clinical Presentation and Conservative Management of Tympanic Membrane Perforation during Intrapartu...