Document Detail


Regulation of adenosine 3',5'-cyclic monophosphate phosphodiesterase activity in fibroblasts by intracellular concentrations of cyclic adenosine monophosphate (3T3-dibutyryl cyclic AMP-SV40-transformed cells-michaelis constants-L cells-prostaglandin E 1 ).
MedLine Citation:
PMID:  4333987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cyclic AMP-phosphodiesterase is present in various mouse fibroblasts. Contact-inhibited 3T3 cells contain two forms of the enzyme, one with a K(m) of 2.5 muM and the second with a K(m) of 71 muM. As 3T3 cells grow to confluency and cAMP concentrations rise, the activity of the first enzyme increases, whereas that of the second is unchanged. A line of SV40-transformed 3T3 cells with low cAMP concentration also has low levels of the cAMP-phosphodiesterase with a K(m) of 2.5 muM. Treatment of 3T3 and SV40-transformed 3T3 cells with dibutyryl cAMP and theophylline increases cAMP-phosphodiesterase accumulation. This accumulation is blocked by cycloheximide and actinomycin D. The newly formed enzyme resembles the higher affinity enzyme present in unstimulated cells, since it has a K(m) of 1.2-2.0 muM, and is stimulated by snake venom. In L cells in which cAMP concentrations are elevated by treatment with prostaglandin E(1), cAMP phosphodiesterase also accumulates. We conclude that intracellular concentrations of cAMP regulate the synthesis of cAMP-phosphodiesterase, and that cAMP functions as an inducer of the enzyme.
Authors:
M D'Armiento; G S Johnson; I Pastan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Feb 
Date Detail:
Created Date:  1972-04-26     Completed Date:  1972-04-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  459-62     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Butyrates / pharmacology
Cell Line
Contact Inhibition
Cyclic AMP / pharmacology*
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Depression, Chemical
Enzyme Activation / drug effects
Enzyme Induction / drug effects
Fibroblasts / drug effects,  enzymology*
Kinetics
L Cells (Cell Line) / enzymology
Mice
Phosphoric Monoester Hydrolases / analysis,  metabolism*
Prostaglandins / pharmacology
Simian virus 40 / cytology,  metabolism
Stimulation, Chemical
Theophylline / pharmacology
Tritium
Chemical
Reg. No./Substance:
0/Butyrates; 0/Prostaglandins; 10028-17-8/Tritium; 50-76-0/Dactinomycin; 58-55-9/Theophylline; 60-92-4/Cyclic AMP; 66-81-9/Cycloheximide; EC 3.1.3.-/Phosphoric Monoester Hydrolases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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