| Regulation of adenosine 3',5'-cyclic monophosphate phosphodiesterase activity in fibroblasts by intracellular concentrations of cyclic adenosine monophosphate (3T3-dibutyryl cyclic AMP-SV40-transformed cells-michaelis constants-L cells-prostaglandin E 1 ). | |
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MedLine Citation:
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PMID: 4333987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyclic AMP-phosphodiesterase is present in various mouse fibroblasts. Contact-inhibited 3T3 cells contain two forms of the enzyme, one with a K(m) of 2.5 muM and the second with a K(m) of 71 muM. As 3T3 cells grow to confluency and cAMP concentrations rise, the activity of the first enzyme increases, whereas that of the second is unchanged. A line of SV40-transformed 3T3 cells with low cAMP concentration also has low levels of the cAMP-phosphodiesterase with a K(m) of 2.5 muM. Treatment of 3T3 and SV40-transformed 3T3 cells with dibutyryl cAMP and theophylline increases cAMP-phosphodiesterase accumulation. This accumulation is blocked by cycloheximide and actinomycin D. The newly formed enzyme resembles the higher affinity enzyme present in unstimulated cells, since it has a K(m) of 1.2-2.0 muM, and is stimulated by snake venom. In L cells in which cAMP concentrations are elevated by treatment with prostaglandin E(1), cAMP phosphodiesterase also accumulates. We conclude that intracellular concentrations of cAMP regulate the synthesis of cAMP-phosphodiesterase, and that cAMP functions as an inducer of the enzyme. |
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Authors:
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M D'Armiento; G S Johnson; I Pastan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 69 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1972 Feb |
Date Detail:
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Created Date: 1972-04-26 Completed Date: 1972-04-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 459-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Butyrates / pharmacology Cell Line Contact Inhibition Cyclic AMP / pharmacology* Cycloheximide / pharmacology Dactinomycin / pharmacology Depression, Chemical Enzyme Activation / drug effects Enzyme Induction / drug effects Fibroblasts / drug effects, enzymology* Kinetics L Cells (Cell Line) / enzymology Mice Phosphoric Monoester Hydrolases / analysis, metabolism* Prostaglandins / pharmacology Simian virus 40 / cytology, metabolism Stimulation, Chemical Theophylline / pharmacology Tritium |
| Chemical | |
Reg. No./Substance:
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0/Butyrates; 0/Prostaglandins; 10028-17-8/Tritium; 50-76-0/Dactinomycin; 58-55-9/Theophylline; 60-92-4/Cyclic AMP; 66-81-9/Cycloheximide; EC 3.1.3.-/Phosphoric Monoester Hydrolases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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