Document Detail

Regulation of activin receptor-interacting protein 2 expression in mouse hepatoma Hepa1-6 cells and its relationship with collagen type IV.
MedLine Citation:
PMID:  17907296     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the regulation of activin receptor-interacting protein 2 (ARIP2) expression and its possible relationships with collagen type IV (collagen IV) in mouse hepatoma cell line Hepal-6 cells.
METHODS: The ARIP2 mRNA expression kinetics in Hepal-6 cells was detected by RT-PCR, and its regulation factors were analyzed by treatment with signal transduction activators such as phorbol 12-myristate 13-acetate (PMA), forskolin and A23187. After pcDNA3-ARIP2 was transfected into Hepal-6 cells, the effects of ARIP2 overexpression on activin type II receptor (ActRII) and collagen IV expression were evaluated.
RESULTS: The expression levels of ARIP2 mRNA in Hapel-6 cells were elevated in time-dependent manner 12 h after treatment with activin A and endotoxin LPS, but not changed evidently in the early stage of stimulation (2 or 4 h). The ARIP2 mRNA expression was increased after stimulated with signal transduction activators such as PMA and forskolin in Hepal-6 cells, whereas decreased after treatment with A23187 (25.3% +/- 5.7% vs 48.1% +/- 3.6%, P < 0.01). ARIP2 overexpression could remarkably suppress the expression of ActRIIA mRNA in dose-dependent manner, but has no effect on ActRIIB in Hepal-6 cells induced by activin A. Furthermore, we have found that overexpression of ARIP2 could inhibit collagen IV mRNA and protein expressions induced by activin A in Hapel-6 cells.
CONCLUSION: These findings suggest that ARIP2 expression can be influenced by various factors. ARIP2 may participate in the negative feedback regulation of signal transduction in the late stage by affecting the expression of ActRIIA and play an important role in regulation of development of liver fibrosis induced by activin.
Hong-Jun Zhang; Gui-Xiang Tai; Jing Zhou; Di Ma; Zhong-Hui Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  13     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-01     Completed Date:  2007-12-20     Revised Date:  2014-09-25    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  5501-5     Citation Subset:  IM    
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MeSH Terms
Activin Receptors, Type II / genetics,  metabolism*
Activins / metabolism
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Adenylate Cyclase / metabolism
Calcimycin / pharmacology
Calcium / metabolism
Carcinoma, Hepatocellular / enzymology,  genetics,  metabolism*
Cell Line, Tumor
Colforsin / pharmacology
Collagen Type IV / genetics,  metabolism*
Enzyme Activators / pharmacology
Gene Expression Regulation, Neoplastic* / drug effects
Ionophores / pharmacology
Lipopolysaccharides / pharmacology
Liver Neoplasms / enzymology,  genetics,  metabolism*
Membrane Proteins / genetics,  metabolism*
Protein Kinase C / metabolism
RNA, Messenger / metabolism
Signal Transduction* / drug effects,  genetics
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Collagen Type IV; 0/Enzyme Activators; 0/Ionophores; 0/Lipopolysaccharides; 0/Membrane Proteins; 0/RNA, Messenger; 0/Synj2bp protein, mouse; 0/activin A; 0/lipopolysaccharide, Escherichia coli 0111 B4; 104625-48-1/Activins; 1F7A44V6OU/Colforsin; 37H9VM9WZL/Calcimycin; EC Kinase C; EC Receptors, Type II; EC receptor type II-A; EC Cyclase; NI40JAQ945/Tetradecanoylphorbol Acetate; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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