Document Detail


Regulation of acetylcholine-induced phosphorylation of PLD1 in porcine tracheal smooth muscle.
MedLine Citation:
PMID:  15591778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The muscarinic agonist, acetylcholine (ACh), stimulates phospholipase D (PLD) activity in tracheal smooth muscle cells. Direct activation of protein kinase C (PKC) by phorbol-12-myristate-13-acetate (PMA) also stimulates PLD in this tissue. Activation of ACh-induced PLD was inhibited by the tyrosine kinase inhibitor genistein in a concentration-dependent manner. Presently known isoforms of PLD, PLD1 and PLD2, were identified in tracheal smooth muscle and their activation-induced phosphorylation status studied. Both ACh and PMA increased phosphorylation of PLD1 that was significantly blocked by genistein or the PKC inhibitor calphostin C. PLD2 phosphorylation was not detected in the present experiments. Western blots probed with an anti-phosphotyrosine antibody indicate that PLD1 in this tissue is phosphorylated on tyrosine residues after ACh or PMA stimulation. Tyrosine phosphorylation of PLD1 was blocked by genistein and calphostin C. No tyrosine residues were phosphorylated on PLD2. Taken together, these results demonstrate that porcine tracheal smooth muscle cells express both isoforms PLD1 and PLD2. However, on muscarinic activation only PLD1 in this tissue is phosphorylated by PKC via a tyrosine-kinase-dependent pathway.
Authors:
Abulkhair M Mamoon; Rodney C Baker; Jerry M Farley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomedical science     Volume:  11     ISSN:  1021-7770     ISO Abbreviation:  J. Biomed. Sci.     Publication Date:    2004 Nov-Dec
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-05-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421567     Medline TA:  J Biomed Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  810-7     Citation Subset:  IM    
Copyright Information:
2004 National Science Council, ROC and S. Karger AG, Basel
Affiliation:
Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Miss. 39216-4505, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / metabolism*
Animals
Blotting, Western
Chromatography, Thin Layer
Dose-Response Relationship, Drug
Genistein / pharmacology
Lipid Metabolism
Male
Muscle, Smooth / cytology*
Muscles / cytology,  metabolism
Myocytes, Smooth Muscle / cytology
Phorbol Esters / chemistry
Phospholipase D / metabolism*
Phosphorylation
Receptors, Muscarinic / metabolism
Swine
Time Factors
Trachea / metabolism*
Tyrosine / chemistry
Grant Support
ID/Acronym/Agency:
HL-55547/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Phorbol Esters; 0/Receptors, Muscarinic; 20839-06-9/phorbol-12-myristate; 446-72-0/Genistein; 51-84-3/Acetylcholine; 55520-40-6/Tyrosine; EC 3.1.4.-/phospholipase D2; EC 3.1.4.4/Phospholipase D; EC 3.1.4.4/phospholipase D1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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