| Regulation of Virulence of Entamoeba histolytica by the URE3-BP Transcription Factor. | |
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MedLine Citation:
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PMID: 20689746 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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It is not understood why only some infections with Entamoeba histolytica result in disease. The calcium-regulated transcription factor upstream regulatory element 3-binding protein (URE3-BP) was initially identified by virtue of its role in regulating the expression of two amebic virulence genes, the Gal/GalNac lectin and ferredoxin. Here we tested whether this transcription factor has a broader role in regulating virulence. A comparison of in vivo to in vitro parasite gene expression demonstrated that 39% of in vivo regulated transcripts contained the URE3 motif recognized by URE3-BP, compared to 23% of all promoters (P < 0.0001). Amebae induced to express a dominant positive mutant form of URE3-BP had an increase in an elongated morphology (30% +/- 6% versus 14% +/- 5%; P = 0.001), a 2-fold competitive advantage at invading the intestinal epithelium (P = 0.017), and a 3-fold increase in liver abscess size (0.1 +/- 0.1 g versus 0.036 +/- 0.1 g; P = 0.03). These results support a role for URE3-BP in virulence regulation. |
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Authors:
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Carol A Gilchrist; Ellyn S Moore; Yan Zhang; Christina B Bousquet; Joanne A Lannigan; Barbara J Mann; William A Petri |
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Publication Detail:
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Type: Journal Article Date: 2010-05-18 |
Journal Detail:
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Title: mBio Volume: 1 ISSN: 2150-7511 ISO Abbreviation: MBio Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2011-01-06 Revised Date: 2013-03-21 |
Medline Journal Info:
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Nlm Unique ID: 101519231 Medline TA: MBio Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: - |
Affiliation:
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Department of Medicine, School of Medicine, University of Virginia, Charlottesville, Virginia, USA. |
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