Document Detail


Regulation of the Vibrio anguillarum metalloprotease EmpA by posttranslational modification.
MedLine Citation:
PMID:  15774867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zinc metalloprotease EmpA is a virulence factor in the fish pathogen Vibrio anguillarum. Previous studies have shown that two strains of V. anguillarum regulate empA differently. Strain M93Sm exhibits protease activity only in the presence of fish gastrointestinal mucus, while protease activity is detected in NB10 culture supernatant under all stationary-phase conditions. In this study, we use real-time reverse transcription-PCR to show that even in conditions where no protease activity is detected, empA transcription occurs. Western blot analysis revealed that EmpA is secreted as a approximately 48-kDa proenzyme and that activation occurs extracellularly by the removal of a approximately 10-kDa peptide. The presence of stable extracellular pro-EmpA in M93Sm culture supernatants suggests that activation of EmpA is not autolytic.
Authors:
Andrew M Staroscik; Steven M Denkin; David R Nelson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  187     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-18     Completed Date:  2005-04-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2257-60     Citation Subset:  IM    
Affiliation:
Department of Cell and Molecular Biology, 117 Morrill Science Building, University of Rhode Island, Kingston, RI 02881. dnelson@uri.edu.
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MeSH Terms
Descriptor/Qualifier:
Gene Expression Regulation, Bacterial
Metalloproteases / genetics,  metabolism*
Protein Processing, Post-Translational / physiology*
Species Specificity
Time Factors
Transcription, Genetic
Vibrio / enzymology*
Chemical
Reg. No./Substance:
EC 3.4.-/Metalloproteases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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