Document Detail

Regulation of VE-cadherin linkage to the cytoskeleton in endothelial cells exposed to fluid shear stress.
MedLine Citation:
PMID:  11822879     Owner:  NLM     Status:  MEDLINE    
Endothelial cells exposed to shear stress realigned and elongated in the direction of flow through the coordinated remodeling of their adherens junctions and actin cytoskeleton. The elaborate networks of VE-cadherin complexes in static cultures became more uniform and compact in response to shear. In contrast, the cortical actin present in static cultures was reorganized into numerous stress fiber bundles distributed parallel to the direction of flow. Exposure to shear did not significantly alter the expression of the junctional proteins VE-cadherin, beta-catenin, and alpha-catenin, but the composition of the junctional complexes did change. We detected a marked decrease in the alpha-catenin associated with VE-cadherin complexes in endothelial monolayers subjected to shear. This loss of alpha-catenin, the protein that links beta-catenin-bound cadherin to the actin cytoskeleton, was not due to decreased quantities of beta-catenin associated with VE-cadherin. Instead, the loss of alpha-catenin from the junctional complexes coincided with the increased tyrosine phosphorylation of beta-catenin associated with VE-cadherin. The change in beta-catenin phosphorylation closely correlated with the shear-induced loss of the protein tyrosine phosphatase SHP-2 from VE-cadherin complexes. Thus, the functional interaction of alpha-catenin with VE-cadherin-bound beta-catenin is regulated by the extent of tyrosine phosphorylation of beta-catenin. This, concomitantly, is regulated by SHP-2 associated with VE-cadherin complexes.
Jon A Ukropec; M Katherine Hollinger; Marilyn J Woolkalis
Related Documents :
7650039 - Identification of plakoglobin domains required for association with n-cadherin and alph...
11526479 - Igf-ii induces rapid beta-catenin relocation to the nucleus during epithelium to mesenc...
17438349 - Immunohistochemical localization of cadherin and catenin adhesion molecules in the muri...
11439369 - Abnormalities of the e-cadherin/catenin adhesion complex in classical papillary thyroid...
16720909 - Type i collagen and divalent cation shifts disrupt cell-cell adhesion, increase migrati...
11043399 - Analysis of cadherin/catenin complexes in transformed thyroid epithelial cells: modulat...
20053759 - The hdac inhibitors trichostatin a and suberoylanilide hydroxamic acid exhibit multiple...
9690209 - Cytotoxic effect of shiga toxin-1 on human proximal tubule cells.
10917949 - Neointimal formation at the sites of anastomosis of the internal thoracic artery grafts...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  273     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-01     Completed Date:  2002-03-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  240-7     Citation Subset:  IM    
Copyright Information:
©2002 Elsevier Science (USA).
Department of Physiology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, CD
Cadherins / biosynthesis,  metabolism*
Cells, Cultured
Cytoskeletal Proteins / metabolism*
Cytoskeleton / metabolism
Endothelium, Vascular / cytology,  metabolism*
Intercellular Junctions / metabolism
Intracellular Signaling Peptides and Proteins
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases / metabolism*
Stress, Mechanical
Tyrosine / metabolism
Umbilical Veins / cytology,  metabolism
alpha Catenin
beta Catenin
Grant Support
Reg. No./Substance:
0/Antigens, CD; 0/CTNNA1 protein, human; 0/CTNNB1 protein, human; 0/Cadherins; 0/Cytoskeletal Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Trans-Activators; 0/alpha Catenin; 0/beta Catenin; 0/cadherin 5; 55520-40-6/Tyrosine; EC protein, human; EC protein, human; EC Tyrosine Phosphatase, Non-Receptor Type 11; EC Tyrosine Phosphatase, Non-Receptor Type 6; EC Tyrosine Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1.
Next Document:  Transforming growth factor beta stimulates fibroblast-collagen matrix contraction by different mecha...