Document Detail


Regulation of Schistocerca gregaria neuroparsin transcript levels by juvenile hormone and 20-hydroxyecdysone.
MedLine Citation:
PMID:  16783827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuroparsins (NPs) are small proteins that were originally discovered in the pars intercerebralis-corpus cardiacum neurosecretory complex of the migratory locust brain. From the desert locust, Schistocerca gregaria, we recently cloned four different transcripts, each coding for a distinct NP-related peptide. In addition to the brain, some NP-like precursor (Scg-NPP) transcripts also occur in a number of peripheral tissues, and their expression levels are controlled in a gender- and stage-dependent manner. Previous studies revealed a close correlation between Scg-NPP transcript levels and the gonotrophic cycle. In the present report, we demonstrate that certain Scg-NPP transcript levels are significantly altered upon injection of juvenile hormone (JH) or 20-hydroxyecdysone (20E) in adult gregarious desert locusts (five days after final ecdysis). While Scg-NPP1 transcript levels did not significantly change as a result of hormone treatment (animals were analyzed 24 h after injection), Scg-NPP2, Scg-NPP3, and Scg-NPP4 displayed hormone-dependent regulation in various tissues. Scg-NPP2 and Scg-NPP3 transcript levels significantly increased in the brain of JH-treated locusts. In addition, JH induction of Scg-NPP3 and Scg-NPP4 transcripts was observed in male fat body and in male and female gonads. Furthermore, 20E injection also induced Scg-NPP2, Scg-NPP3, and Scg-NPP4 transcripts in desert locust gonads. This is the first report showing NP-like precursor gene expression in insect ovaries. Our study indicates that the expression levels of some Scg-NPP transcripts are regulated by developmental hormones, suggesting a close correlation between NP expression and the endocrine control of the reproductive cycle.
Authors:
Ilse Claeys; Bert Breugelmans; Gert Simonet; Vanessa Franssens; Sofie Van Soest; Jozef Vanden Broeck
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of insect biochemistry and physiology     Volume:  62     ISSN:  0739-4462     ISO Abbreviation:  Arch. Insect Biochem. Physiol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-26     Completed Date:  2006-08-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8501752     Medline TA:  Arch Insect Biochem Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  107-15     Citation Subset:  IM    
Affiliation:
Laboratory of Developmental Physiology, Genomics and Proteomics, K.U. Leuven, Leuven, Belgium. Ilse.Claeys@bio.kuleuven.be
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MeSH Terms
Descriptor/Qualifier:
Actins / biosynthesis
Animals
DNA Primers / chemistry
Ecdysterone / pharmacology*
Female
Gene Expression / drug effects*
Gene Expression Profiling / methods
Grasshoppers / physiology*
Injections
Insect Hormones / biosynthesis,  genetics,  physiology*
Juvenile Hormones / pharmacology*
Male
Reverse Transcriptase Polymerase Chain Reaction / methods
Chemical
Reg. No./Substance:
0/Actins; 0/DNA Primers; 0/Insect Hormones; 0/Juvenile Hormones; 5289-74-7/Ecdysterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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