Document Detail


Regulation of the SM-20 prolyl hydroxylase gene in smooth muscle cells.
MedLine Citation:
PMID:  15081411     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
SM-20 encodes an intracellular prolyl hydroxylase that acts on hypoxia inducible factor (HIF)-1alpha, targeting it for proteasomal degradation. By decreasing HIF-alpha, SM-20 is thought to modulate the expression of hypoxia-regulated genes. SM-20 expression in the arterial wall is restricted to smooth muscle cells, which play a critical role in atherosclerosis and arterial injury. To further elucidate the regulation of SM-20 in smooth muscle, we cloned and analyzed the rat SM-20 promoter. In transient transfections, the SM-20 promoter displayed approximately 6-fold greater activity in smooth muscle cells vs. fibroblasts. Deletion analysis and electrophoretic mobility shift assays demonstrated that SM-20 transcription was regulated by two Sp1/Sp3 sites. A shift in binding to the Sp1/Sp3 sites, a decrease in Sp1 and Sp3 protein levels, and the emergence of a lower molecular weight form of Sp1 were seen in serum-deprived or post-confluent SMC, suggesting that SM-20 is regulated during smooth muscle cell differentiation.
Authors:
Keon Menzies; Bin Liu; William J H Kim; Maria C Moschella; Mark B Taubman
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  317     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-14     Completed Date:  2004-05-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  801-10     Citation Subset:  IM    
Affiliation:
The Zena and Michael A. Wiener Cardiovascular Institute, Department of Medicine, The Mount Sinai School of Medicine, New York, NY 10029, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA
Electrophoretic Mobility Shift Assay
Molecular Sequence Data
Muscle, Smooth, Vascular / cytology,  metabolism*
Mutation
Procollagen-Proline Dioxygenase / genetics*
Promoter Regions, Genetic
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
F31 GM15732/GM/NIGMS NIH HHS; R01 HL43302/HL/NHLBI NIH HHS; T32 HL07824/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 1.14.11.2/Procollagen-Proline Dioxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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