| Regulation of RNA splicing in gag-deficient mutants of Moloney murine sarcoma virus MuSVts110. | |
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MedLine Citation:
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PMID: 2157036 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated whether the MuSVts110 gag gene product (P58gag) can regulate the novel growth temperature dependence of MuSVts110 RNA splicing. MuSVts110 mutants with either frameshifts or deletions in the gag gene were tested for their ability to maintain the MuSVts110 splicing phenotype. Only small decreases in splicing efficiency and no changes in the thermosensitivity of viral RNA splicing were observed in MuSVts110 gag gene frameshift mutants. Deletions within the gag gene, however, variably decreased MuSVts110 splicing efficiency but had no effect on its thermosensitivity. Another class of MuSVts110 splicing mutants generated by treatment of MuSVts110-infected cells with NiCl2 was also examined. In these "nickel revertants," P58gag is made, but splicing of the viral transcript is nearly complete at all growth temperatures. The splicing of "tagged" viral RNA transcribed from a modified MuSVts110 DNA introduced into nickel revertant cells remained thermosensitive, arguing against trans effects of viral gene products on splicing efficiency. These experiments indicated that neither the MuSVts110 P58gag protein nor any other viral gene product acts in trans to regulate MuSVts110 splicing. |
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Authors:
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M De Mars; D A Sterner; S M Chiocca; N W Biggart; E C Murphy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 64 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1990 Apr |
Date Detail:
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Created Date: 1990-05-03 Completed Date: 1990-05-03 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1421-8 Citation Subset: IM; X |
Affiliation:
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Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Gene Expression Regulation, Viral / genetics, physiology* Gene Products, gag / genetics, physiology* Genes, gag / genetics Moloney murine sarcoma virus / drug effects, genetics*, growth & development Mutagens / pharmacology Mutation* Nickel / pharmacology RNA Splicing / genetics* RNA, Viral / genetics*, metabolism Sarcoma Viruses, Murine / genetics* Virus Cultivation |
| Grant Support | |
ID/Acronym/Agency:
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5T32 CA 0929-11/CA/NCI NIH HHS; CA-16672/CA/NCI NIH HHS; CA-34734/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gene Products, gag; 0/Mutagens; 0/RNA, Viral; 7440-02-0/Nickel |
| Comments/Corrections | |
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