| Regulation of RAD54- and RAD52-lacZ gene fusions in Saccharomyces cerevisiae in response to DNA damage. | |
| | |
MedLine Citation:
|
PMID: 3550429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The RAD52 and RAD54 genes in the yeast Saccharomyces cerevisiae are involved in both DNA repair and DNA recombination. RAD54 has recently been shown to be inducible by X-rays, while RAD52 is not. To further investigate the regulation of these genes, we constructed gene fusions using 5' regions upstream of the RAD52 and RAD54 genes and a 3'-terminal fragment of the Escherichia coli beta-galactosidase gene. Yeast transformants with either an integrated or an autonomously replicating plasmid containing these fusions expressed beta-galactosidase activity constitutively. In addition, the RAD54 gene fusion was inducible in both haploid and diploid cells in response to the DNA-damaging agents X-rays, UV light, and methyl methanesulfonate, but not in response to heat shock. The RAD52-lacZ gene fusion showed little or no induction in response to X-ray or UV radiation nor methyl methanesulfonate. Typical induction levels for RAD54 in cells exposed to such agents were from 3- to 12-fold, in good agreement with previous mRNA analyses. When MATa cells were arrested in G1 with alpha-factor, RAD54 was still inducible after DNA damage, indicating that the observed induction is independent of the cell cycle. Using a yeast vector containing the EcoRI structural gene fused to the GAL1 promoter, we showed that double-strand breaks alone are sufficient in vivo for induction of RAD54. |
| | |
Authors:
|
G M Cole; D Schild; S T Lovett; R K Mortimer |
Related Documents
:
|
8978689 - Requirement for pp1 phosphatase and 20s cyclosome/apc for the onset of anaphase is less... 8675019 - Amiloride toxicity in the fission yeast schizosaccharomyces pombe is released by thiami... 8973339 - Expression of the major capsid protein of human papillomavirus type 11 in saccharomyces... 9606189 - Nascent transcription from the nmt1 and nmt2 genes of schizosaccharomyces pombe overlap... 12455959 - The mold-specific ms8 gene is required for normal hypha formation in the dimorphic path... 15692559 - The transcriptional coactivator pc4/sub1 has multiple functions in rna polymerase ii tr... 19704679 - Enemy at the gates: interaction-specific stomatal responses to pathogenic challenge. 11960329 - Myeloid differentiation (myd)/growth arrest dna damage (gadd) genes in tumor suppressio... 9335309 - Temporal regulation of sigd from bacillus subtilis depends on a minor promoter in front... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Molecular and cellular biology Volume: 7 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1987 Mar |
Date Detail:
|
Created Date: 1987-05-18 Completed Date: 1987-05-18 Revised Date: 2010-09-10 |
Medline Journal Info:
|
Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1078-84 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle DNA Damage DNA Repair* Gene Expression Regulation Genes, Fungal* / drug effects, radiation effects Hot Temperature Methyl Methanesulfonate Plasmids SOS Response (Genetics) Saccharomyces cerevisiae / drug effects, genetics*, radiation effects Transformation, Genetic Ultraviolet Rays |
| Grant Support | |
ID/Acronym/Agency:
|
GM07127/GM/NIGMS NIH HHS; GM30990/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
66-27-3/Methyl Methanesulfonate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nucleotide sequence and functional analysis of the RAD1 gene of Saccharomyces cerevisiae.
Next Document: Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galacto...