Document Detail

Regulation of PTEN expression in neuronal apoptosis.
MedLine Citation:
PMID:  10581415     Owner:  NLM     Status:  MEDLINE    
PTEN phosphatase is a tumor suppressor gene that dephosphorylates phosphatidylinositol phosphates. PTEN restrains the function of a major antiapoptotic and survival pathway involving phosphoinositide 3-kinase and Akt kinase. Our purpose was to find out whether apoptotic inducers affect the expression of PTEN in cerebellar granule neurons and neuroblastoma 2a cells (Neuro-2a). PTEN mRNA expression showed a major 5.5-kb and a lower abundance 2.5-kb transcripts. In Neuro-2a cells, serum withdrawal induced a prominent, continuous decrease both in 5.5- and 2.5-kb transcripts of PTEN mRNA. Simultaneously, the expression level of 56-kDa PTEN protein decreased in Neuro-2a cells. The decrease in PTEN expression precedes apoptotic changes observed after serum withdrawal. On the contrary, okadaic acid and etoposide only slightly affected the expression of PTEN although they induce a prominent apoptosis in Neuro-2a cells. In cerebellar granule neurons, okadaic acid treatment induced a prominent increase in PTEN mRNA expression after 6-h treatment, both at the 5.5- and 2.5-kb transcripts. The early response in PTEN mRNA expression disappeared in 5.5-kb transcripts already at 12 h and in the case of 2.5-kb transcripts it lasted up to 24 h. Potassium deprivation, known to induce apoptosis in cerebellar granule cells, did not affect PTEN mRNA expression but together with serum deprivation induced a clear decrease in the 5. 5-kb PTEN transcripts. It seems that the changes in PTEN expression level and neuronal apoptosis are not related to each other in general but the expression of PTEN phosphatase seems to regulate certain apoptotic signals affecting phosphoinositide 3-kinase function.
S Kyrylenko; M Roschier; P Korhonen; A Salminen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  73     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-02-14     Completed Date:  2000-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  198-202     Citation Subset:  IM    
Department of Neuroscience and Neurology, University of Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland.
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MeSH Terms
Apoptosis / drug effects,  genetics*
Cerebellum / cytology,  drug effects,  enzymology
Culture Media, Serum-Free / pharmacology
Etoposide / pharmacology
Gene Expression Regulation, Enzymologic / drug effects
Neurons / cytology,  drug effects,  enzymology*
Okadaic Acid / pharmacology
PTEN Phosphohydrolase
Phosphoric Monoester Hydrolases / genetics*
RNA, Messenger / drug effects,  genetics,  metabolism
Rats, Wistar
Tumor Cells, Cultured
Tumor Suppressor Proteins*
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/RNA, Messenger; 0/Tumor Suppressor Proteins; 33419-42-0/Etoposide; 78111-17-8/Okadaic Acid; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC Phosphohydrolase

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