Document Detail

Regulation of the PI3-K/Akt Survival Pathway in the Rat Endometrium.
MedLine Citation:
PMID:  23390163     Owner:  NLM     Status:  Publisher    
The occurrence of apoptosis and cell survival in the receptive uterus is intimately involved in the embryo implantation process in order to facilitate embryo attachment to the maternal endometrium. The initial stimulus leading to successful implantation might be triggered by the conceptus itself. By the end of rat embryo implantation, decidualisation begins followed by the regression of the decidua basalis (DB) on Day 14. The PI3-K survival pathway and TGF-beta have been thought to play a role in this process. The objective of the present study was to investigate the regulation of phosphatidylinositol 3-kinase (PI3-K)/PTEN/Akt pathway in the rat endometrium during pregnancy. Rats were killed at different days of pregnancy (Day 1 to 22 and postpartum) or pseudopregnancy (Day 1 to 9) and uteri were removed to collect endometrial tissues. The active form of Akt (pAkt) was increased at Day 5 of pregnancy and at Day 3 of pseudopregnancy as well as at Day 12 of pregnancy and at Day 1 postpartum. Of the three Akt isoforms (Akt1, Akt2 and Akt3), Akt3 was the only isoform phosphorylated at Day 5 during the implantation process and postpartum time as demonstrated by immunoprecipitation studies. PI3-K inhibition in vivo blocked Akt phosphorylation, reduced Smad2 phosphorylation and reduced both TGF-beta2 and XIAP expression. PI3-K inhibition in cultured decidual cells leaded to inhibition of pAkt and decrease XIAP expression. These results suggests that Akt and XIAP may be important surviving signaling molecules by which apoptosis is regulated in the rat endometrium during pregnancy and that TGF-beta could be linked to this process.
Annabelle Veillette; Kathy Grenier; Kevin Brasseur; Guylaine Fréchette-Frigon; Valérie Leblanc; Sophie Parent; Eric Asselin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-6
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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