| Regulation of NeuroD expression by activation of the protein kinase-C pathway in Y79 human retinoblastoma cells. | |
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MedLine Citation:
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PMID: 10507539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To examine the signals that regulate NeuroD expression, we analyzed the effects of activation of two major signal pathways, the protein kinase A (PKA) pathway and the protein kinase C (PKC) pathway, on the expression of NeuroD in Y79human retinoblastoma cells. Activation of PKC resulted in marked induction of NeuroD mRNA and NeuroD protein. NeuroD mRNA induction was inhibited by calphostin C, an inhibitor of PKC. On the other hand, stimulation of PKA by forskolin had a weak suppressive effect on NeuroD mRNA expression. Induction of NeuroD expression was followed by enhancement of expression of the AK1 gene, one of the target genes of NeuroD, which encodes adenylate kinase isozyme 1, an important enzyme in the cellular adenine nucleotide homeostasis. Our results indicate that NeuroD expression is regulated, at least in part, by the PKC pathway and not by the PKA pathway. |
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Authors:
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T Noma; Y S Yoon; Y Yamashiro; K Fujisawa; A Nakazawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience letters Volume: 272 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-11-09 Completed Date: 1999-11-09 Revised Date: 2009-09-04 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 45-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan. tnoma@po.cc.yamaguchi-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Kinase
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drug effects*,
metabolism Basic Helix-Loop-Helix Transcription Factors Cyclic AMP-Dependent Protein Kinases / drug effects*, metabolism Enzyme Activation / drug effects Forskolin / pharmacology Humans Isoenzymes / drug effects*, metabolism Nerve Tissue Proteins / drug effects, metabolism* Protein Kinase C / drug effects*, metabolism RNA, Messenger / drug effects, metabolism* Retinoblastoma Signal Transduction / drug effects, physiology Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Transcription Factors; 0/Isoenzymes; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 16561-29-8/Tetradecanoylphorbol Acetate; 169238-82-8/NeuroD protein; 66428-89-5/Forskolin; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases; EC 2.7.11.13/Protein Kinase C; EC 2.7.4.3/Adenylate Kinase; EC 2.7.4.3/adenylate kinase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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