Document Detail

Regulation of Neural Progenitor Cell Proliferation by D609: Potential Role for ERK.
MedLine Citation:
PMID:  23275176     Owner:  NLM     Status:  Publisher    
Tricyclodecan-9-yl-xanthogenate (D609) has been shown to possess both neuroprotective and anti-proliferative properties. We investigated the role of D609 in reducing the proliferation of neural progenitor cells in vitro. D609 decreased the expression of cyclin D1 after 1 day but not 2 or 4 days in culture, indicating the possible degradation/inactivation of drug in the medium. Consistent with this notion, spectral analysis showed the maximum absorbance of D609 (100 μM) at 300 nm, which decreased by ~30 % following incubation at 37 °C for 24 h. Further experiments revealed that incubation of neural progenitor cells with D609 decreased the phosphorylation of extracellular signal-regulated kinase (ERK) but not Akt. In addition, increasing the concentration of B27 (1-4 %), but not FGF2, diminished the effect of D609 on cell proliferation. These results together suggest that D609 may curtail the proliferation of neural progenitor cells by decreasing the ERK-mediated expression of cyclin D1 and may have a therapeutic potential in containing the proliferation of tumor stem cells.
Haviryaji S G Kalluri; Anchal Gusain; Robert J Dempsey
Related Documents :
24511466 - A panel of cpg methylation sites distinguishes human embryonic stem cells and induced p...
23119226 - Benefits of hypoxic culture on bone marrow multipotent stromal cells.
23405896 - Th17 cell development: from the cradle to the grave.
24431896 - 8-bromo-7-methoxychrysin inhibits properties of liver cancer stem cells via downregulat...
3940256 - Deficient cell-mediated cytotoxicity and hyporesponsiveness to interferon and mitogenic...
23871606 - Reprogramming of human fibroblasts to pluripotency with lineage specifiers.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-30
Journal Detail:
Title:  Molecular neurobiology     Volume:  -     ISSN:  1559-1182     ISO Abbreviation:  Mol. Neurobiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8900963     Medline TA:  Mol Neurobiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Neurological Surgery, University of Wisconsin, Madison, WI, 53792, USA,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  FGF-2 Low Molecular Weight Selectively Promotes Neuritogenesis of Motor Neurons In Vitro.
Next Document:  Allogeneic Stem Cell Transplantation for Chronic Myeloid Leukemia.