Document Detail


Regulation of muscle genes by moderate exercise.
MedLine Citation:
PMID:  20589593     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Moderate-intensity exercise at the lactate threshold (LT) is considered to be a safe and effective training regimen for improving metabolic syndrome. The aim of the current study was to investigate the effects of moderate exercise performed at the LT on skeletal muscle gene expression. 6 healthy men participated in cycle ergometer training at LT, 60 min/d, 5 d/wk for 12 wks. Muscle samples were collected after 5 d of training, and then 2 d after training at wks 6 and 12. Quantitative real-time PCR analysis revealed that the expression of peroxisome proliferator activated receptor co-activated 1alpha was significantly increased at 1 h after the training session on day 5. Moreover, using serial analysis gene expression, we found that moderate training for 6 and 12 wks simultaneously induced the expression of a number of metabolic genes involved in the TCA cycle, beta-oxidation, and electron transport. Furthermore, several genes encoding antioxidant enzymes and contractile apparatus were induced. The expression levels of 233 novel transcripts were also altered in response to moderate exercise. Thus, moderate training at the LT is a sufficient stimulus to induce the expression of numerous genes implicated in the development of metabolic syndrome, transcripts involved in the contractile apparatus, and novel transcripts.
Authors:
Y Nishida; H Tanaka; T Tobina; K Murakami; N Shono; M Shindo; W Ogawa; M Yoshioka; J St-Amand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-29
Journal Detail:
Title:  International journal of sports medicine     Volume:  31     ISSN:  1439-3964     ISO Abbreviation:  Int J Sports Med     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008349     Medline TA:  Int J Sports Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  656-70     Citation Subset:  IM    
Copyright Information:
Georg Thieme Verlag KG Stuttgart . New York.
Affiliation:
Saga University, Department of Preventive Medicine, Faculty of Medicine,Saga, Japan. ynishida@cc.saga-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Anaerobic Threshold / physiology
Bicycling / physiology*
Ergometry
Exercise / physiology
Gene Expression Regulation / physiology*
Humans
Lactic Acid / blood
Male
Muscle, Skeletal / physiology*
Polymerase Chain Reaction
Young Adult
Chemical
Reg. No./Substance:
50-21-5/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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